Novel beta-defensins
AssigneeZymoGenetics, Inc.
InventorsDavid A. Adler, James L. Holloway, Nand Baindur, Stephanie Beigel-Orme, Paul O. Sheppard
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Abstract
The present invention relates to polynucleotide and polypeptide molecules for zamp1, a novel member of the β-defensin family. The polypeptides, and polynucleotides encoding them, exhibit anti-microbial activity and may be used in the study or treatment of microbial infections. The present invention also includes antibodies to the zamp1 polypeptides.
Claims65
An isolated protein comprising a polypeptide that is at least 80% identical to a polypeptide selected from the group consisting of:
- a) a polypeptide having the sequence of amino acid residue 1 to amino acid residue 65 of SEQ ID NO:2;
- b) a polypeptide having the sequence of amino acid residue 19 to amino acid residue 65 of SEQ ID NO:2;
- c) a polypeptide having the sequence of amino acid residue 21 to amino acid residue 65 of SEQ ID NO:2;
- d) a polypeptide having the sequence of amino acid residue 1 to amino acid residue 67 of SEQ ID NO:10;
- e) a polypeptide having the sequence of amino acid residue 21 to amino acid residue 67 of SEQ ID NO:10;
- and
- f) a polypeptide having the sequence of amino acid residue 23 to amino acid residue 67 of SEQ ID NO:2;
- wherein said polypeptide has cysteine residues corresponding to amino acid residues 33, 40, 45, 55, 62 and 63 of SEQ ID NOs:2 or 10.
A polypeptide selected from the group consisting of:
- a) amino acid residue 30 to amino acid residue 63 of SEQ ID NO:2;
- b) amino acid residue 31 to amino acid residue 63 of SEQ ID NO:2;
- c) amino acid residue 30 to amino acid residue 64 of SEQ ID NO:2;
- d) amino acid residue 31 to amino acid residue 64 of SEQ ID NO:2;
- and
- e) a polypeptide chosen from SEQ ID NOs:14-72.
An isolated polynucleotide molecule encoding a protein, said polynucleotide molecule consisting of a coding strand and a complementary non-coding strand, wherein said polynucleotide molecule encodes a polypeptide that is at least 80% identical to the amino acid sequence to a polypeptide selected from the group consisting of:
- a) a polypeptide having the sequence of amino acid residue 1 to amino acid residue 65 of SEQ ID NO:2;
- b) a polypeptide having the sequence of amino acid residue 19 to amino acid residue 65 of SEQ ID NO:2;
- c) a polypeptide having the sequence of amino acid residue 21 to amino acid residue 65 of SEQ ID NO:2;
- d) a polypeptide having the sequence of amino acid residue 1 to amino acid residue 67 of SEQ ID NO:10;
- e) a polypeptide having the sequence of amino acid residue 21 to amino acid residue 67 of SEQ ID NO:10;
- and
- f) a polypeptide having the sequence of amino acid residue 23 to amino acid residue 67 of SEQ ID NO:2;
- wherein said polypeptide has cysteine residues corresponding to amino acid residues 33, 40, 45, 55, 62 and 63 of SEQ ID NOs:2 or 10.
An isolated polynucleotide molecule encoding a protein having cysteine residues corresponding to amino acid residues 33, 40, 45, 55, 62 and 63 of SEQ ID NO:10, said polynucleotide molecule consisting of a coding strand and a complementary non-coding strand, wherein said polynucleotide comprises a nucleotide sequence that is at least 80% identical to the sequence of a polynucleotide selected from the group consisting of:
- a) a polynucleotide as shown in SEQ ID NO:9 from nucleotide 220 to nucleotide 420;
- b) a polynucleotide as shown in SEQ ID NO:9 from nucleotide 280 to nucleotide 420;
- and
- c) a polynucleotide as shown in SEQ ID NO:9 from nucleotide 286 to nucleotide 420.
An isolated polynucleotide molecule encoding a protein having cysteine residues corresponding to amino acid residues 33, 40, 45, 55, 62 and 63 of SEQ ID NO:10, said polynucleotide molecule consisting of a coding strand and a complementary non-coding strand, wherein said polynucleotide comprises a nucleotide sequence as shown in SEQ ID NO:11.
An isolated polynucleotide molecule encoding a polypeptide selected from the group consisting of:
- a) amino acid residue 30 to amino acid residue 63 of SEQ ID NO:2;
- b) amino acid residue 31 to amino acid residue 63 of SEQ ID NO:2;
- c) amino acid residue 30 to amino acid residue 64 of SEQ ID NO:2;
- d) amino acid residue 31 to amino acid residue 64 of SEQ ID NO:2;
- and
- e) a polypeptide chosen from SEQ ID NOs:14-72.
An isolated polynucleotide molecule selected from the group consisting of:
- a) nucleotide 88 to nucleotide 189 of SEQ ID NO:1;
- b) nucleotide 88 to nucleotide 192 of SEQ ID NO:1;
- c) nucleotide 91 to nucleotide 189 of SEQ ID NO:1;
- d) nucleotide 91 to nucleotide 192 of SEQ ID NO:1;
- e) nucleotide 88 to nucleotide 189 of SEQ ID NO:4;
- f) nucleotide 88 to nucleotide 192 of SEQ ID NO:4;
- g) nucleotide 91 to nucleotide 189 of SEQ ID NO:4 and
- h) nucleotide 91 to nucleotide 192 of SEQ ID NO:4.
An oligonucleotide probe or primer comprising at least 14 contiguous nucleotides of a polynucleotide of SEQ ID NO:11 or a sequence complementary to SEQ ID NO:11.
A method of treating a microbial-related disease comprising administering to a mammal a therapeutically effective amount of a polypeptide selected from the group consisting of:
- a) a polypeptide of SEQ ID NO:2;
- b) a polypeptide of SEQ ID NO:10;
- c) a polypeptide chosen from SEQ ID NOs:14-72;
- d) amino acid residue 30 to amino acid residue 63 of SEQ ID NO:2;
- e) amino acid residue 31 to amino acid residue 63 of SEQ ID NO:2;
- f) amino acid residue 30 to amino acid residue 64 of SEQ ID NO:2;
- g) amino acid residue 31 to amino acid residue 64 of SEQ ID NO:2;
- h) amino acid residue 20 to amino acid residue 67 of SEQ ID NO:10 and
- i) amino acid residue 22 to amino acid residue 67 of SEQ ID NO:10;
- whereby said polypeptide ameliorates said disease.
A method of contraception comprising administering to a mammal a therapeutically effective amount of a polypeptide selected from the group consisting of:
- a) a polypeptide of SEQ ID NO:2;
- b) a polypeptide of SEQ ID NO:10;
- c) a polypeptide chosen from SEQ ID NOs:14-72;
- d) amino acid residue 30 to amino acid residue 63 of SEQ ID NO:2;
- e) amino acid residue 31 to amino acid residue 63 of SEQ ID NO:2;
- f) amino acid residue 30 to amino acid residue 64 of SEQ ID NO:2;
- g) amino acid residue 31 to amino acid residue 64 of SEQ ID NO:2;
- h) amino acid residue 20 to amino acid residue 67 of SEQ ID NO:10 and
- i) amino acid residue 22 to amino acid residue 67 of SEQ ID NO:10.
An isolated polypeptide comprising a sequence of amino acid residues having at least 95 percent sequence identity with amino acid residues 31 to 63 of SEQ ID NO:2, wherein the polypeptide has antimicrobial activity.
An isolated polypeptide consisting of a sequence of amino acid residues having at least 95 percent sequence identity with amino acid residues 31 to 63 of SEQ ID NO:2, wherein the polypeptide has antimicrobial activity.
An isolated polypeptide comprising a sequence of amino acid residues having at least 95 percent sequence identity with amino acid residues 23 to 67 of SEQ ID NO:10, wherein the polypeptide has antimicrobial activity.
An isolated polypeptide consisting of a sequence of amino acid residues having at least 95 percent sequence identity with amino acid residues 23 to 67 of SEQ ID NO:10, wherein the polypeptide has antimicrobial activity.
An isolated polypeptide comprising an amino acid sequence of SEQ ID NO:2.
An isolated polypeptide comprising amino acid residues 21 to 65 of SEQ ID NO:2.
An isolated polypeptide comprising amino acid residues 23 to 67 of SEQ ID NO:10.
An isolated polypeptide comprising an amino acid sequence of SEQ ID NO:10.
Dependencies are inferred from the claim text.