Mammalian neuro-growth factor like protein
AssigneeZymoGenetics, Inc.
InventorsPaul O. Sheppard, Laura J. Jelinek
Life of this patent
Estimating the term…
Abstract
Novel mammalian Zneu1 polypeptides, polynucleotides encoding the polypeptides, and related compositions and methods including antibodies and anti-idiotypic antibodies.
Claims15
An isolated polynucleotide which encodes a mammalian Zneu1 polypeptide wherein said polynucleotide encodes a polypeptide selected from the group SEQ ID NOs:2-3,8, 9, 11-16, and 19-24 or a polypeptide which is at least 90% identical to the polypeptides of said group and which retain the activity of said polypeptides.
An isolated polynucleotide which encodes a peptide or polypeptide having at least 15 amino acid residues comprised of an epitope-bearing portion of a polypeptide of SEQ ID NOs:
- 2-3,8, 9, 11-16, and 19-24 or a polypeptide which is at least 90% identical to said polypeptides.
An expression vector comprising the following operably linked elements:
- a transcription promoter;
- a DNA segment which encodes a Zneu1 polypeptide or a peptide or polypeptide which contains an epitope-bearing region of a Zneu1 polypeptide;
- and a transcription terminator.
An expression vector comprising the following operably linked elements:
- (a) a transcription promoter;
- (b) a DNA segment encoding a chimeric polypeptide, wherein said chimeric polypeptide consists essentially of a first portion and a second portion joined by a peptide bond, said first portion being comprised of a mammalian polypeptide, said polypeptide being the amino acid sequences of SEQ ID NOs:
- 2-3,8, 9, 11-16, and 19-24 and said second portion being a second polypeptide or protein.
- (c) a transcription terminator.
An isolated Zneu1 polypeptide selected from the group of amino acid sequences consisting of SEQ ID NOs:
- 2-3,8, 9, 11-16, and 19-24 or a polypeptide which is at least 90% identical to said polypeptides.
An isolated peptide or polypeptide having at least 15 amino acid residues comprised of an epitope-bearing portion of a polypeptide of SEQ ID NOs:
- 2-3,8, 9, 11-16, and 19-24 or is at least 90% identical to said epitope bearing portion.
An antibody, antibody fragment or single-chain antibody that specifically binds to a mammalian polypeptide, said polypeptide being defined by the amino acid sequences of SEQ ID NOs:
- 2-3,8, 9, 11-16, and 19-24.
A method for producing an antibody which binds to a peptide or polypeptide defined by SEQ ID NOs:
- 2-3,8, 9, 11-16, and 19-24 or to a peptide or polypeptide which is at least 90% identical to said peptide or polypeptide comprising inoculating an animal with said peptide or polypeptide or with a nucleic acid which encodes said peptide or polypeptide, wherein said animal produces antibodies to said peptide or polypeptide;
- and
- isolating said antibody.
An anti-idiotypic antibody, anti-idiotypic antibody fragment or anti-idiotypic single-chain antibody which binds to an antibody, an antibody fragment or single-chain antibody of peptide or polypeptide defined by SEQ ID NOs:
- 2-3,8, 9, 11-16, and 19-24 or to a peptide or polypeptide which is at least 90% identical to said peptide or polypeptide
Dependencies are inferred from the claim text.