Back to results
US 2003/0166898 A1Published application
Myelin oligodendrocyte glycoprotein-like protein (MOGp)
AssigneeHuman Genome Sciences, Inc.
InventorsArvind Chopra, Henrik S. Olsen, Reiner L. Gentz, Steven M. Ruben
Life of this patent
Estimating the term…
Abstract
The present invention relates to a novel MOGp protein which is a member of the Ig superfamily. In particular, isolated nucleic acid molecules are provided encoding the human MOGp protein. MOGp polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. Also provided are diagnostic and therapeutic methods for detecting and treating cancer, inflammation, and multiple sclerosis (MS).
Claims35
An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) a nucleotide sequence encoding the MOGp polypeptide having the complete amino acid sequence in FIG. 1 (SEQ ID NO:2);
- (b) a nucleotide sequence encoding the MOGp polypeptide having the amino acid sequence at positions 2-331 in FIG. 1 (SEQ ID NO:2);
- (c) a nucleotide sequence encoding the mature MOGp polypeptide having the amino acid sequence at positions 30-331 in FIG. 1 (SEQ ID NO:2);
- (d) a nucleotide sequence encoding the MOGp polypeptide having the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97709;
- (e) a nucleotide sequence encoding the mature MOGp polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97709;
- (f) a nucleotide sequence encoding the MOGp polypeptide extracellular domain having an amino acid sequence at positions 30 to 247 shown in FIG. 1 [SEQ ID NO:2];
- (g) a nucleotide sequence encoding the MOGp polypeptide transmembrane domain having an amino acid sequence at positions 248 to 271 shown in FIG. 1 [SEQ ID NO:2];
- (h) a nucleotide sequence encoding the MOGp polypeptide intracellular domain having an amino acid sequence at positions 272 to 331 shown in FIG. 1 [SEQ ID NO:2];
- (i) a nucleotide sequence encoding the MOGp receptor extracellular and intracellular domains with all or part of the transmembrane domain deleted;
- and
- (j) a nucleotide sequence complementary to any of the nucleotide sequences in (a), (b), (c), (d), (e), (f), (g), (h), or (i).
8 more elements
An isolated nucleic acid molecule comprising a polynucleotide having a sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) the nucleotide sequence of a fragment of the sequence shown in SEQ ID NO:1, wherein said fragment comprises at least 50 contiguous nucleotides of SEQ ID NO:1, provided that said nucleotide sequence is not HAFAV34R (SEQ ID NO. 11);
- HETBC89R (SEQ ID. NO. 12);
- HRDDL76R (SEQ ID. NO. 13);
- HRDDL35R (SEQ ID NO. 14);
- HRDDI47R (SEQ ID NO. 15);
- HRDDK16R (SEQ ID NO. 16);
- HRDDK03R (SEQ ID NO. 17);
- HRDDK54R (SEQ ID NO. 18);
- HRDBQ91R (SEQ ID NO. 19);
- HRDCB31R (SEQ ID NO. 20);
- HRDDL95R (SEQ ID NO. 21);
- HFCAE49F (SEQ ID NO. 22);
- HTWAL13R (SEQ ID NO. 23);
- T91685 (SEQ ID NO. 24);
- AA303854 (SEQ ID NO. 25);
- T70127 (SEQ ID NO. 26);
- T86577 (SEQ ID NO. 27);
- AA337675 (SEQ ID NO. 28);
- T94934 (SEQ ID NO. 29);
- AA114263 (SEQ ID NO. 30);
- T92875 (SEQ ID NO. 31);
- AA484820 (SEQ ID NO. 32);
- T70246 (SEQ ID NO. 33);
- AA134341 (SEQ ID NO. 34);
- AA134342 (SEQ ID NO. 35);
- T94480 (SEQ ID NO. 36);
- T89056 (SEQ ID NO. 37);
- T86754 (SEQ ID NO. 38);
- and T98146 (SEQ ID NO. 39) or any subfragment thereof;
- and
- (b) a nucleotide sequence complementary to a nucleotide sequence in (a).
28 more elements
An isolated MOGp polypeptide having an amino acid sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) the amino acid sequence of the MOGp polypeptide having the complete amino acid sequence in FIG. 1 (SEQ ID NO:2);
- (b) the amino acid sequence of the MOGp polypeptide having the amino acid sequence at positions 2-331 in FIG. 1 (SEQ ID NO:2);
- (c) the amino acid sequence of the mature MOGp polypeptide having the amino acid sequence at positions 30-331 in FIG. 1 (SEQ ID NO:2);
- (d) the amino acid sequence of the MOGp polypeptide having the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97709;
- (e) the amino acid sequence of the mature MOGp polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97709;
- and
- (f) the amino acid sequence of the MOGp extracellular domain having an amino acid sequence at positions 30 to 250 shown in FIG. 1;
- (g) the amino acid sequence of the MOGp transmembrane domain having an amino acid sequence at positions 251 to 270 shown in FIG. 1;
- (h) the amino acid sequence of the MOGp intracellular domain having an amino acid sequence at positions 271 to 331 shown in FIG. 1;
- and
- (i) the amino acid sequence of an epitope-bearing portion of any one of the polypeptides of (a), (b), (c), (d), (e), (f), (g), or (h).
8 more elements
An isolated polypeptide comprising an epitope-bearing portion of the MOGp protein, wherein said portion is selected from the group consisting of:
- a polypeptide comprising amino acid residues from about 1 to about 125 in FIG. 1 (SEQ ID NO:2);
- a polypeptide comprising amino acid residues from about 1 to about 55 in FIG. 1 (SEQ ID NO:2);
- a polypeptide comprising amino acid residues from about 1 to about 55 in FIG. 1 (SEQ ID NO:2);
- a polypeptide comprising amino acid residues from about 80 to about 113 in FIG. 1 (SEQ ID No:2);
- a polypeptide comprising amino acid residues from about 282 to about 297 in FIG. 1 (SEQ ID No:2);
- a polypeptide comprising amino acid residues from about 299 to about 331 in FIG. 1 (SEQ ID No:2);
- a polypeptide comprising amino acid residues from about 46 to about 53 in FIG. 1 (SEQ ID No:2);
- a polypeptide comprising amino acid residues from about 59 to about 65 in FIG. 1 (SEQ ID No:2);
- a polypeptide comprising amino acid residues from about 71 to about 77 in FIG. 1 (SEQ ID No:2);
- a polypeptide comprising amino acid residues from about 119 to about 125 in FIG. 1 (SEQ ID No:2);
- a polypeptide comprising amino acid residues from about 130 to about 137 in FIG. 1 (SEQ ID No:2);
- a polypeptide comprising amino acid residues from about 183 to about 190 in FIG. 1 (SEQ ID No:2);
- a polypeptide comprising amino acid residues from about 211 to about 219 in FIG. 1 (SEQ ID No:2);
- a polypeptide comprising amino acid residues from about 239 to about 248 in FIG. 1 (SEQ ID No:2);
- and a polypeptide comprising amino acid residues from about 275 to about 280 in FIG. 1 (SEQ ID No:2).
12 more elements
A method of diagnosing multiple sclerosis comprising:
- (a) providing a biological sample from an individual to be tested for multiple sclerosis;
- (b) assaying said biological sample for the amount of antibody to MOGp protein present in said biological sample;
- (c) comparing the amount of antibody to MOGp protein in said biological sample to the amount of antibody to MOGp protein in a standard sample from an individual not having multiple sclerosis;
- and
- (d) correlating an enhanced amount of the antibody in said biological sample relative to said standard with an increased probability of multiple sclerosis.
2 more elements
A method used for the diagnosis of a tumor or inflammatory disease, comprising:
- (a) assaying MOGp protein gene expression level in mammalian cells or body fluid;
- and
- (b) comparing said MOGp protein gene expression level with a standard MOGp protein gene expression level whereby an increase in said MOGp gene expression level over said standard is indicative of an increased probability of a tumor or inflammatory disease.
An isolated nucleic acid molecule comprising a MOGp structural gene operably linked to a heterologous promoter.
Dependencies are inferred from the claim text.