Method of processing a proteinaceous material to recover K-casein macropeptide and polymers of a-lactalbumin and B-lactoglobulin
AssigneeLand O' Lakes, Inc.
InventorErnest P. Brody
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Abstract
A method of processing a proteinaceous material that includes κ-casein macropeptide, the method entailing polymerizing protein present in the proteinaceous material to yield a proteinaceous intermediate, where the proteinaceous intermediate includes polymerized protein, and separating the proteinaceous intermediate to yield a first portion and a second portion, where the first portion includes a majority of the κ-casein macropeptide from the proteinaceous material and the second portion includes a majority of the polymerized protein from the proteinaceous intermediate.
Claims60
A method of fractionating a first proteinaceous material or a second proteinaceous material, the method comprising:
- heating the first proteinaceous material to an elevated temperature to yield a third proteinaceous material, the first proteinaceous material comprising κ-casein macropeptide and a heat labile protein, the pH of the first proteinaceous material being greater than about 7, and the elevated temperature effective to polymerize at least some of the heat labile protein present in the first proteinaceous material;
- or
- providing the second proteinaceous material with a pH greater than about 7 to yield the third proteinaceous material, the second proteinaceous material comprising κ-casein macropeptide and the heat labile protein, the temperature of the second proteinaceous material being the elevated temperature, and the elevated temperature effective to polymerize at least some of the heat labile protein present in the second proteinaceous material;
- cooling the third proteinaceous material to yield a fourth proteinaceous material;
- and
- passing the fourth proteinaceous material across a filtration membrane to yield a filtration retentate and a filtration permeate, the filtration permeate comprising a majority of the κ-casein macropeptide from the first proteinaceous material or a majority of the κ-casein macropeptide from the second proteinaceous material.
A method of processing a proteinaceous material, the proteinaceous material comprising κ-casein macropeptide, the method comprising:
- polymerizing protein present in the proteinaceous material to yield a proteinaceous intermediate, the proteinaceous intermediate comprising polymerized protein;
- and
- separating the proteinaceous intermediate to yield a first portion and a second portion, the first portion comprising a majority of the κ-casein macropeptide from the proteinaceous material and the second portion comprising a majority of the polymerized protein from the proteinaceous intermediate.
A method of fractionating a proteinaceous material, the proteinaceous material comprising κ-casein macropeptide and heat labile protein, the method comprising:
- providing the proteinaceous material with a combination of a pH, an elevated temperature, and a heated holding period that is effective to cause polymerization of at least some of the heat labile protein and yield a proteinaceous intermediate that comprises polymerized protein;
- and
- separating the proteinaceous intermediate to yield a first portion and a second portion, the first portion comprising at least about 80 weight percent of the κ-casein macropeptide from the proteinaceous material and the second portion comprising at least about 80 weight percent of the polymerized protein from the proteinaceous intermediate.
A method of fractionating a proteinaceous material, the proteinaceous material comprising κ-casein macropeptide and heat labile protein, the method comprising:
- providing the proteinaceous material with a combination of a pH, an elevated temperature, and a heated holding period that is effective to cause polymerization of at least some of the heat labile protein and yield a proteinaceous intermediate that comprises polymerized protein;
- and
- separating the proteinaceous intermediate to yield a first portion and a second portion, the first portion comprising κ-casein macropeptide from the proteinaceous material and the second portion comprising at least about 80 weight percent of the polymerized protein from the proteinaceous intermediate, the first portion and the proteinaceous material, when each subjected to the same reversed-phase high performance liquid chromatography analysis under identical analysis conditions, exhibiting the same, or essentially the same, κ-casein macropeptide profile.
A polymerized protein material, the polymerized protein material comprising polymerized β-lactoglobulin or polymerized α-lactalbumin, the polymerized protein material when mixed with water in a ratio of about 10 grams of the polymerized protein material to about 90 grams of water to form an aqueous solution, is soluble in the aqueous solution when the aqueous solution has a temperature of about 72° F.
A polymerized protein material, the polymerized protein material comprising polymers of β-lactoglobulin and α-lactalbumin, the polymerized protein material, when mixed with water in a ratio of about 10 grams of the polymerized protein material to about 90 grams of water to form an aqueous solution, effective to provide the aqueous solution with a Brookfield viscosity of at least about 6,000 centipoise when the temperature of the aqueous solution is about 72° F.
A κ-casein macropeptide-enriched powder, the κ-casein macropeptide-enriched powder comprising protein, the protein comprising β-lactoglobulin or α-lactalbumin and the protein further comprising κ-casein macropeptide, the κ-casein macropeptide-enriched powder having a protein concentration of at least about 75 weight percent, based upon the total weight of the κ-casein macropeptide-enriched powder, the concentration of κ-casein macropeptide in the κ-casein macropeptide-enriched powder being at least about 35 weight percent, based upon the total weight of protein in the κ-casein macropeptide-enriched powder, and the weight ratio of κ-casein macropeptide to the collective weight of β-lactoglobulin and α-lactalbumin in the κ-casein macropeptide-enriched powder being at least about 0.7.
Dependencies are inferred from the claim text.