Human blue-light photoreceptor hCRY2
AssigneeHuman Genome Sciences, Inc.
InventorsYing-Fei Wei, Steven M. Ruben, Aziz Sancar, Shiao-Wen D. Hsu, Aleksey G. Kazantsev
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Abstract
The present invention relates to a novel member of the blue-light photoreceptor family of receptors. In particular, isolated nucleic acid molecules are provided encoding the human hCRY2 receptor. hCRY2 polypeptides are also provided as are vectors, host cells, antibodies, and recombinant methods for producing the same.
Claims18
An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) a nucleotide sequence encoding the amino acid sequence at position −22 to 571 in SEQ ID NO:2;
- (b) a nucleotide sequence encoding the amino acid sequence at position −21 to 571 in SEQ ID NO:2;
- (c) a nucleotide sequence encoding the amino acid sequence at position 1 to 571 in SEQ ID NO:2;
- (d) a nucleotide sequence encoding the amino acid sequence at position 191 to 571 in SEQ ID NO:2;
- (e) a nucleotide sequence encoding the amino acid sequence at 1 to 492 in SEQ ID NO:2;
- (f) a nucleotide sequence encoding the amino acid sequence at 493 to 505 in SEQ ID NO:2;
- (g) a nucleotide sequence encoding the amino acid sequence at 506 to 571 in SEQ ID NO:2;
- (h) a nucleotide sequence encoding the hCRY2 receptor having the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97769;
- (i) a nucleotide sequence encoding the mature hCRY2 receptor having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97769;
- and
- (j) a nucleotide sequence complementary to any of the nucleotide sequences in (a), (b), (c), (d), (e), (f), (g), (h), or (i).
An isolated nucleic acid molecule, comprising a polynucleotide having a sequence selected from the group consisting of:
- (a) the nucleotide sequence of a fragment of the sequence shown in SEQ ID NO:1, wherein said fragment comprises at least 50 contiguous nucleotides of SEQ ID NO:1, provided that said fragment is not SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, or SEQ ID NO:22 or any subfragment thereof;
- and
- (b) a nucleotide sequence complementary to a nucleotide sequence in (a).
An isolated polypeptide comprising an amino acid sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) amino acids −22 to 571 in SEQ ID NO:2;
- (b) amino acids −21 to 571 in SEQ ID NO:2;
- (c) amino acids 1 to 571 in SEQ ID NO:2;
- (d) amino acids 191 to 571 in SEQ ID NO:2;
- (e) amino acids 1 to 492 in SEQ ID NO:2;
- (f) amino acids 493 to 505 in SEQ ID NO:2;
- (g) amino acids 506 to 571 in SEQ ID NO:2;
- (h) the amino acid sequence of the hCRY2 polypeptide having the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97769;
- (i) the amino acid sequence of the mature hCRY2 polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No.97769;
- and
- (j) the amino acid sequence of an epitope-bearing portion of any one of the polypeptides of (a), (b), (c), (d), (e), (f), (g), (h), or (i).
An isolated polypeptide comprising an epitope-bearing portion of the hCRY2 receptor protein, wherein said portion is selected from the group consisting of:
- a polypeptide comprising amino acid residues from about 8 to about 17 in SEQ ID NO:2;
- a polypeptide comprising amino acid residues from about 23 to about 30 in SEQ ID NO:2;
- a polypeptide comprising amino acid residues from about 55 to about 70 in SEQ ID NO:2;
- a polypeptide comprising amino acid residues from about 74 to about 81 in SEQ ID NO:2;
- a polypeptide comprising amino acid residues from about 136 to about 147 in SEQ ID NO:2;
- a polypeptide comprising amino acid residues from about 156 to about 165 in SEQ ID NO:2;
- a polypeptide comprising amino acid residues from about 231 to about 239 in SEQ ID NO:2;
- a polypeptide comprising amino acid residues from about 271 to about 279 in SEQ ID NO:2;
- a polypeptide comprising amino acid residues from about 298 to about 309 in SEQ ID NO:2;
- a polypeptide comprising amino acid residues from about 316 to about 324 in SEQ ID NO:2;
- a polypeptide comprising amino acid residues from about 328 to about 334 in SEQ ID NO:2;
- a polypeptide comprising amino acid residues from about 415 to about 426 in SEQ ID NO:2;
- and a polypeptide comprising amino acid residues from about 512 to about 519 in SEQ ID NO:2.
An isolated nucleic acid molecule comprising a polynucleotide encoding a hCRY2 polypeptide wherein, except for one to fifty conservative amino acid substitutions, said polypeptide has a sequence selected from the group consisting of:
- (a) a nucleotide sequence encoding the amino acid sequence at position −22 to 571 in SEQ ID NO:2;
- (b) a nucleotide sequence encoding the amino acid sequence at position −21 to 571 in SEQ ID NO:2;
- (c) a nucleotide sequence encoding the amino acid sequence at position 1 to 571 in SEQ ID NO:2;
- (d) a nucleotide sequence encoding the amino acid sequence at position 191 to 571 in SEQ ID NO:2;
- (e) a nucleotide sequence encoding the amino acid sequence at 1 to 492 in SEQ ID NO:2;
- (f) a nucleotide sequence encoding the amino acid sequence at 493 to 505 of SEQ ID NO:2;
- (g) a nucleotide sequence encoding the amino acid sequence at 506 to 571 of SEQ ID NO:2;
- (h) a nucleotide sequence encoding the hCRY2 receptor having the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97769;
- (i) a nucleotide sequence encoding the mature hCRY2 receptor having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97769;
- and
- (j) a nucleotide sequence complementary to any of the nucleotide sequences in (a), (b), (c), (d), (e), (f), (g), (h), or (i).
An isolated hCRY2 polypeptide wherein, except for one to fifty conservative amino acid substitutions, said polypeptide has a sequence selected from the group consisting of:
- (a) amino acids −22 to 571 in SEQ ID NO:2;
- (b) amino acids −21 to 571 in SEQ ID NO:2;
- (c) amino acids 1 to 571 in SEQ ID NO:2;
- (d) amino acids 191 to 571 in SEQ ID NO:2;
- (e) amino acids 1 to 492 in SEQ ID NO:2;
- (f) amino acids 493 to 505 in SEQ ID NO:2;
- (g) amino acids 506 to 571 in SEQ ID NO:2;
- (h) the amino acid sequence of the hCRY2 polypeptide having the complete amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97769;
- (i) the amino acid sequence of the mature hCRY2 polypeptide having the amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No.97769;
- and
- (j) the amino acid sequence of an epitope-bearing portion of any one of the polypeptides of (a), (b), (c), (d), (e), (f), (g), (h), or (i).
Dependencies are inferred from the claim text.