US 2003/0166855 A1Published application

Lipoxygenase polynucleotides and methods of use

AssigneePioneer Hi-Bred International, Inc.

InventorsPedro A. Navarro Acevedo, Jonathan P. Duvick, Mikhailo V. Kolomiets, Carl R. Simmons

Life of this patent

Estimating the term…

Abstract

Methods and compositions for modulating development and defense response are provided. Nucleotide sequences encoding maize lipoxygenase proteins and a lipoxygenase gene promoter region are provided. The sequences can be used in expression cassettes for modulating development, developmental pathways, and the plant defense response. Transformed plants, plant cells, tissues, and seed are also provided.

Claims17

Dependent claims

An isolated polypeptide selected from the group consisting of:

(a) a polypeptide comprising an amino acid sequence set forth in SEQ ID NO:
6, 8, 14, 16, 18, 20, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 50, or 52;
(b) a polypeptide encoded by a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
5, 7, 13, 15, 17, 19, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 49, or 51;
(c) a polypeptide encoded by a nucleotide sequence that hybridizes under stringent conditions to a nucleotide sequence comprising the sequences set forth in SEQ ID NO:
5, 7, 13, 15, 17, 19, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 49, or 51;
(d) an amino acid sequence comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
6 or 8, wherein said sequence has LOX-like activity;
(e) an amino acid sequence comprising an amino acid sequence having at least 84% sequence identity to the sequence set forth in SEQ ID NO:
14 or 16, wherein said sequence has LOX-like activity;
(f) an amino acid sequence comprising an amino acid sequence having at least 75% sequence identity to the sequence set forth in SEQ ID NO:
18 or 20, wherein said sequence has LOX-like activity;
(g) an amino acid sequence comprising an amino acid sequence having at least 50% sequence identity to the sequence set forth in SEQ ID NO:
26 or 28, wherein said sequence has LOX-like activity;
(h) an amino acid sequence comprising an amino acid sequence having at least 65% sequence identity to the sequence set forth in SEQ ID NO:
30 or 32, wherein said sequence has LOX-like activity;
(i) an amino acid sequence comprising an amino acid sequence having at least 59% sequence identity to the sequence set forth in SEQ ID NO:
34 or 36, wherein said sequence has LOX-like activity;
(j) an amino acid sequence comprising an amino acid sequence having at least 70% sequence identity to the sequence set forth in SEQ ID NO:
38 or 40, wherein said sequence has LOX-like activity;
(k) an amino acid sequence comprising an amino acid sequence having at least 69% identity to the sequence set forth in SEQ ID NO:
42 or 44, wherein said sequence has LOX-like activity;
and
(l) an amino acid sequence comprising an amino acid sequence having at least 52% identity to the sequence set forth in SEQ ID NO:
50 or 52, wherein said sequence has LOX-like activity.

22 more elements

An isolated nucleic acid molecule selected from the group consisting of:

(a) a nucleic acid molecule comprising a nucleotide sequence set forth in SEQ ID NO:
5, 7, 13, 15, 17, 19, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 49, or 51;
(b) a nucleic acid molecule comprising a nucleotide sequence encoding an amino acid sequence set forth in SEQ ID NO:
6, 8, 14, 16, 18, 20, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 50, or 52;
(c) a nucleic acid molecule comprising an antisense sequence corresponding to a sequence of a) or b);
(d) a nucleic acid molecule comprising a nucleotide sequence that hybridizes under stringent conditions to the nucleotide sequences of a), b), or c), wherein said sequence encodes a polypeptide having LOX-like activity;
(e) a nucleic acid molecule comprising a nucleotide sequence having at least 60% sequence identity to the sequence set forth in SEQ ID NO:
13, 17, 25, 27, 29, 31, 33, 35, 37, 41, 49, or 51, wherein said sequence encodes a polypeptide having LOX-like activity;
(f) a nucleic acid molecule comprising a nucleotide sequence having at least 98% sequence identity to the sequence set forth in SEQ ID NO:
7, wherein said sequence encodes a polypeptide having LOX-like activity;
(g) a nucleic acid molecule comprising a nucleotide sequence having at least 90% sequence identity to the sequence set forth in SEQ ID NO:
15, wherein said sequence encodes a polypeptide having LOX-like activity;
(h) a nucleic acid molecule comprising a nucleotide sequence having at least 81% sequence identity to the sequence set forth in SEQ ID NO:
19, wherein said sequence encodes a polypeptide having LOX-like activity;
(i) a nucleic acid molecule comprising a nucleotide sequence having at least 65% sequence identity to the sequence set forth in SEQ ID NO:
39, wherein said sequence encodes a polypeptide having LOX-like activity;
(j) a nucleic acid molecule comprising a nucleotide sequence having at least 66% sequence identity to the sequence set forth in SEQ ID NO:
43, wherein said sequence encodes a polypeptide having LOX-like activity;
and
(k) a nucleic acid molecule comprising a nucleotide sequence having at least 95% sequence identity to the sequence set forth in SEQ ID NO:
5, wherein said sequence encodes a polypeptide having LOX-like activity.

18 more elements

A cell having stably incorporated into its genome at least one DNA construct comprising a nucleotide sequence operably linked to a promoter that drives expression in said cell, wherein said nucleotide sequence is selected from the group consisting of:

(a) a nucleic acid molecule comprising a nucleotide sequence set forth in SEQ ID NO:
5, 7, 13, 15, 17, 19, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 49, or 51;
(b) a nucleic acid molecule comprising a nucleotide sequence encoding an amino acid sequence set forth in SEQ ID NO:
6, 8, 14, 16, 18, 20, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 50, or 52;
(c) a nucleic acid molecule comprising an antisense sequence corresponding to a sequence of a) or b);
(d) a nucleic acid molecule comprising a nucleotide sequence that hybridizes under stringent conditions to the nucleotide sequences of a), b), or c), wherein said sequence encodes a polypeptide having LOX-like activity;
(e) a nucleic acid molecule comprising a nucleotide sequence having at least:
60% sequence identity to the sequence set forth in SEQ ID NO:
13, 17, 25, 27, 29, 31, 33, 35, 37, 41, 49, or 51, wherein said sequence encodes a polypeptide having LOX-like activity;
(f) a nucleic acid molecule comprising a nucleotide sequence having at least 98% sequence identity to the sequence set forth in SEQ ID NO:
7, wherein said sequence encodes a polypeptide having LOX-like activity;
(g) a nucleic acid molecule comprising a nucleotide sequence having at least 90% sequence identity to the sequence set forth in SEQ ID NO:
15, wherein said sequence encodes a polypeptide having LOX-like activity;
(h) a nucleic acid molecule comprising a nucleotide sequence having at least 81% sequence identity to the sequence set forth in SEQ ID NO:
19, wherein said sequence encodes a polypeptide having LOX-like activity;
(i) a nucleic acid molecule comprising a nucleotide sequence having at least 65% sequence identity to the sequence set forth in SEQ ID NO:
39, wherein said sequence encodes a polypeptide having LOX-like activity;
(j) a nucleic acid molecule comprising a nucleotide sequence having at least 66% sequence identity to the sequence set forth in SEQ ID NO:
43, wherein said sequence encodes a polypeptide having LOX-like activity;
and
(k) a nucleic acid molecule comprising a nucleotide sequence having at least 95% sequence identity to the sequence set forth in SEQ ID NO:
5, wherein said sequence encodes a polypeptide having LOX-like activity.

19 more elements

A transformed plant having stably incorporated into its genome at least one DNA construct comprising a nucleotide sequence operably linked to a heterologous promoter that drives expression in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:

(a) a nucleic acid molecule comprising a nucleotide sequence set forth in SEQ ID NO:
5, 7, 13, 15, 17, 19, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 49, or 51;
(b) a nucleic acid molecule comprising a nucleotide sequence encoding an amino acid sequence set forth in SEQ ID NO:
6, 8, 14, 16, 18, 20, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 50, or 52;
(c) a nucleic acid molecule comprising an antisense sequence corresponding to a sequence of a) or b);
(d) a nucleic acid molecule comprising a nucleotide sequence that hybridizes under stringent conditions to the nucleotide sequences of a), b), or c), wherein said sequence encodes a polypeptide having LOX-like activity;
(e) a nucleic acid molecule comprising a nucleotide sequence having at least:
60% sequence identity to the sequence set forth in SEQ ID NO:
13, 17, 25, 27, 29, 31, 33, 35, 37, 41, 49, or 51, wherein said sequence encodes a polypeptide having LOX-like activity;
(f) a nucleic acid molecule comprising a nucleotide sequence having at least 98% sequence identity to the sequence set forth in SEQ iID NO:
7, wherein said sequence encodes a polypeptide having LOX-like activity;
(g) a nucleic acid molecule comprising a nucleotide sequence having at least 90% sequence identity to the sequence set forth in SEQ ID NO:
15, wherein said sequence encodes a polypeptide having LOX-like activity;
(h) a nucleic acid molecule comprising a nucleotide sequence having at least 81% sequence identity to the sequence set forth in SEQ ID NO:
19, wherein said sequence encodes a polypeptide having LOX-like activity;
(i) a nucleic acid molecule comprising a nucleotide sequence having at least 65% sequence identity to the sequence set forth in SEQ ID NO:
39, wherein said sequence encodes a polypeptide having LOX-like activity;
(j) a nucleic acid molecule comprising a nucleotide sequence having at least 66% sequence identity to the sequence set forth in SEQ ID NO:
43, wherein said sequence encodes a polypeptide having LOX-like activity;
and
(k) a nucleic acid molecule comprising a nucleotide sequence having at least 95% sequence identity to the sequence set forth in SEQ ID NO:
5, wherein said sequence encodes a polypeptide having LOX-like activity.

19 more elements

A method for enhancing a plant defense response, said method comprising stably introducing into the genome of a plant at least one DNA construct comprising a nucleotide sequence operably linked to a heterologous promoter that drives expression in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:

(a) a nucleic acid molecule comprising a nucleotide sequence set forth in SEQ ID NO:
5, 7, 13, 15, 17, 19, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 49, or 51;
(b) a nucleic acid molecule comprising a nucleotide sequence encoding an amino acid sequence set forth in SEQ ID NO:
6, 8, 14, 16, 18, 20, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 50, or 52;
(c) a nucleic acid molecule comprising an antisense sequence corresponding to a sequence of a) or b);
(d) a nucleic acid molecule comprising a nucleotide sequence that hybridizes under stringent conditions to the nucleotide sequences of a), b) or c), wherein said sequence encodes a polypeptide having LOX-like activity;
(e) a nucleic acid molecule comprising a nucleotide sequence having at least 60% sequence identity to the sequence set forth in SEQ ID NO:
13, 17, 25, 27, 29, 31, 33, 35, 37, 41, 49, or 51, wherein said sequence encodes a polypeptide having LOX-like activity;
(f) a nucleic acid molecule comprising a nucleotide sequence having at least 98% sequence identity to the sequence set forth in SEQ ID NO:
7, wherein said sequence encodes polypeptide having LOX-like activity;
(g) a nucleic acid molecule comprising a nucleotide sequence having at least 90% sequence identity to the sequence set forth in SEQ ID NO:
15, wherein said sequence encodes polypeptide having LOX-like activity;
(h) a nucleic acid molecule comprising a nucleotide sequence having at least 81% sequence identity to the sequence set forth in SEQ If) NO:
19, wherein said sequence encodes polypeptide having LOX-like activity;
(i) a nucleic acid molecule comprising a nucleotide sequence having at least 65% sequence identity to the sequence set forth in SEQ ID NO:
39, wherein said sequence encodes polypeptide having LOX-like activity;
(j) a nucleic acid molecule comprising a nucleotide sequence having at least 66% sequence identity to the sequence set forth in SEQ ID NO:
43, wherein said sequence encodes a polypeptide having LOX-like activity;
and
(k) a nucleic acid molecule comprising a nucleotide sequence having at least 95% sequence identity to the sequence set forth in SEQ ID NO:
5, wherein said sequence encodes a polypeptide having LOX-like activity.

18 more elements

Dependencies are inferred from the claim text.

Facts

Application: 10/132,350Filed 25 Apr 2002
Priority: 27 Apr 2001
Published: 4 Sep 2003
Term ends: Not estimatedThe source records don’t support an estimate.

Classification5 codes

C12N 9/0069
Oxidoreductases: acting on single donors with incorporation of molecular oxygen, i.e. oxygenases
C07K 14/415
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from plants
C07K 2319/00
Fusion polypeptide
C12N
Microorganisms or enzymes

Family

Application60/286,889Us provisional application · filed 27 Apr 2001
Application60/305,366Us provisional application · filed 13 Jul 2001
US 6,921,847 B2Related patent
US 2003/0166855 A1This patent

From the US filing history. Foreign family members aren’t listed.

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Abstract

An isolated polypeptide selected from the group consisting of:

(a) a polypeptide comprising an amino acid sequence set forth in SEQ ID NO:
6, 8, 14, 16, 18, 20, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 50, or 52;
(b) a polypeptide encoded by a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
5, 7, 13, 15, 17, 19, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 49, or 51;
(c) a polypeptide encoded by a nucleotide sequence that hybridizes under stringent conditions to a nucleotide sequence comprising the sequences set forth in SEQ ID NO:
5, 7, 13, 15, 17, 19, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 49, or 51;
(d) an amino acid sequence comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
6 or 8, wherein said sequence has LOX-like activity;
(e) an amino acid sequence comprising an amino acid sequence having at least 84% sequence identity to the sequence set forth in SEQ ID NO:
14 or 16, wherein said sequence has LOX-like activity;
(f) an amino acid sequence comprising an amino acid sequence having at least 75% sequence identity to the sequence set forth in SEQ ID NO:
18 or 20, wherein said sequence has LOX-like activity;
(g) an amino acid sequence comprising an amino acid sequence having at least 50% sequence identity to the sequence set forth in SEQ ID NO:
26 or 28, wherein said sequence has LOX-like activity;
(h) an amino acid sequence comprising an amino acid sequence having at least 65% sequence identity to the sequence set forth in SEQ ID NO:
30 or 32, wherein said sequence has LOX-like activity;
(i) an amino acid sequence comprising an amino acid sequence having at least 59% sequence identity to the sequence set forth in SEQ ID NO:
34 or 36, wherein said sequence has LOX-like activity;
(j) an amino acid sequence comprising an amino acid sequence having at least 70% sequence identity to the sequence set forth in SEQ ID NO:
38 or 40, wherein said sequence has LOX-like activity;
(k) an amino acid sequence comprising an amino acid sequence having at least 69% identity to the sequence set forth in SEQ ID NO:
42 or 44, wherein said sequence has LOX-like activity;
and
(l) an amino acid sequence comprising an amino acid sequence having at least 52% identity to the sequence set forth in SEQ ID NO:
50 or 52, wherein said sequence has LOX-like activity.

22 more elements

Facts

Application

10/132,350Filed 25 Apr 2002

Priority

27 Apr 2001

Published

4 Sep 2003

Term ends

Not estimatedThe source records don’t support an estimate.

Classification5 codes

C12N 9/0069
Oxidoreductases: acting on single donors with incorporation of molecular oxygen, i.e. oxygenases
C07K 14/415
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from plants
C07K 2319/00
Fusion polypeptide
C12N
Microorganisms or enzymes