DNA which encodes trehalase and uses thereof
AssigneeKabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
InventorsYoshiaki Yanai, Harumi Ariyasu, Tsunetaka Ohta, Masashi Kurimoto
Life of this patent
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Abstract
Disclosed are a DNA which encodes murine trehalase, a polypeptide expressed by the DNA, and a transgenic- and knockout-animals which have been genetically engineered with the DNA. The DNA comprises a part or the whole of the nucleotide sequence of SEQ ID NO:1.
Claims8
A polypeptide which is obtainable by the expression of a DNA which comprises the nucleotide sequence of SEQ ID NO:1 that encodes a trehalase or a fragment thereof.
A process for producing polypeptide, which comprises:
- allowing a transformed cell, which has been obtained by introducing a DNA, which comprises the nucleotide sequence of SEQ ID NO:1 that encodes a trehalase or a fragment thereof, into an appropriate host cell derived from a microorganism, plant or animal including insect, to express a polypeptide encoded by the DNA;
- and
- collecting the expressed polypeptide, wherein said polypeptide comprises a part or the whole of the amino acid sequence of SEQ ID NO:2.
A transgenic animal, into which a part or the whole of the nucleotide sequence of SEQ ID NO:1 has been introduced.
A knockout animal, which a trehalase gene, comprising a part or the whole of the nucleotides sequence SEQ ID NO:1, has been destroyed.
Dependencies are inferred from the claim text.