C/EBP beta isoforms and methods of use in cell regulation and anti-tumorigenesis
OwnerLinda Sealyinventor
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Abstract
The present invention relates generally to biological methods for treating tumors, including metastatic tumors. More particularly, the present invention discloses aberrant signal transduction pathways related to tumorigenesis. The present invention provides compositions and methods for treating tumors and improving the condition of a mammal in need thereof. The present invention further provides compositions and methods for regulation of cellular functions including, but not limited to: proliferation, differentiation, and cell death.
Claims41
A method of treating a tumor in a mammal in need thereof, comprising administering an anti-tumor effective amount of an isolated C/EBPβ isoform to the mammal.
A kit for treating a tumor in a mammal in need thereof, comprising one or more suitable containers holding an amount of an isolated C/EBPβ isoform polypeptide and a set of instructions for administering the isolated C/EBPβ isoform to the mammal, wherein the isolated C/EBPβ isoform polypeptide is an isolated C/EBPβ-1 isoform polypeptide or a C/EBP3 isoform polypeptide, and wherein the isolated C/EBPβ isoform polypeptide is substantially free of a C/EBPβ-2 isoform polypeptide.
A kit for treating a tumor in a mammal in need thereof, comprising one or more suitable containers holding an amount of an isolated expression vector including an expression insert having a polynucleotide segment encoding a C/EBPβ isoform, wherein the expression vector includes at least one genetic control element operatively linked to the expression insert for driving an expression of the C/EBPβ isoform in a cell of the mammal.
A method of inhibiting tumorigenesis in a population of cells in a mammal comprising administering an effective amount of an isolated C/EBPβ isoform to the population of cells in the mammal, wherein the isolated C/EBPβ isoform is an isolated C/EBPβ-1 isoform or an isolated C/EBPβ-3 isoform.
A method of inhibiting proliferation of a mammalian cell, comprising administering an effective amount of an isolated C/EBPβ isoform to the mammalian cell, wherein the isolated C/EBPβ isoform is an isolated C/EBPβ-1 isoform or an isolated C/EBPβ-3 isoform.
A method of inducing differentiation of a mammalian cell, comprising introducing an effective amount of an isolated C/EBPβ-1 isoform to the mammalian cell.
A method of promoting a cell death of a mammalian cell, comprising administering an effective amount of an isolated C/EBPβ-3 isoform to the mammalian cell.
A composition comprising an isolated polynucleotide encoding a C/EBPβ-1 isoform having a mutation which eliminates a C/EBPβ-2 isoform production site.
A composition comprising an isolated polypeptide including a membrane transport sequence operably linked to a C/EBPβ-1 isoform polypeptide or a C/EBPβ-3 isoform polypeptide.
A method of manufacturing an anti-tumor agent comprising the steps of:
- a. obtaining a polynucleotide sequence encoding a C/EBPβ-1 isoform of a C/EBPβ;
- b. modifying the polynucleotide sequence encoding the C/EBPβ-1 isoform to prevent a production of a C/EBPβ-2 isoform;
- c. combining the modified polynucleotide sequence encoding the C/EBPβ-1 isoform with a nucleic acid capable of expressing a C/EBPβ-1 isoform polypeptide in a cell;
- and
- d. expressing the C/EBPβ-1 isoform polypeptide in the cell.
A method of making an anti-tumor agent comprising the steps of:
- a. obtaining a polynucleotide sequence encoding a C/EBPβ-3 isoform of a C/EBPβ;
- b. combining the modified polynucleotide sequence encoding the C/EBPβ-1 isoform with a nucleic acid capable of expressing a C/EBPβ-1 isoform polypeptide in a cell;
- and
- c. expressing the C/EBPβ-1 isoform polypeptide in the cell.
Dependencies are inferred from the claim text.