Synthetic immunogenic but non-deposit-forming polypeptides and peptides homologous to amyloid beta, prion protein, amylin, alpha-synuclein, or polyglutamine repeats for induction of an immune response thereto

An isolated polypeptide or peptide represented by:

• (A) SEQ ID NO:32;
• (B) a segment of SEQ ID NO:32, comprising residues 90 to 144 of SEQ ID NO:32, alone or in which either a polyaspartate or polylysine of 4 to 10 residues in length is joined to said segment at the N-terminus and/or C-terminus of said segment;
• or
• (C) the formula
• (A)m—(N—Xaa1Xaa2GlyGlyLeuGlyGlyXaa3Xaa4Xaa5—C)n—(B)p
• wherein:
• m is 0, 4, 5, 6, 7, 8, 9, or 10;
• p is 0, 4, 5, 6, 7, 8, 9, or 10;
• A is Lys or Asp;
• B is Lys or Asp;
• n is 1 or 2;
• N is residues 90-120 of SEQ ID NO:21;
• C is residues 131-144 of SEQ ID NO:21;
• Xaa1, Xaa2, Xaa3, Xaa4, and Xaa5 are Val, Val, Tyr, Met, and Leu, respectively, in which zero or one to five of residues Xaa1, Xaa2, Xaa3, Xaa4, and Xaa5 is substituted with Pro, Asp, Glu, Lys, Gly, or Ser;
• and
• when zero residue is substituted, then either or both of m and p is not zero.

An isolated polypeptide or peptide represented by:

• (A) SEQ ID NO:33;
• (B) a segment of the sequence of SEQ ID NO:33, comprising residues 93 to 156 of SEQ ID NO:33, alone or in which either a polyaspartate or polylysine of 4 to 10 residues in length is joined to said segment at the N-terminus and/or C-terminus of said segment;
• or
• (C) the formula
• (A)m—(N—Xaa1Xaa2GlyGlyLeuGlyGlyXaa3Xaa4Xaa5—C)n—(B)p
• wherein:
• m is 0, 4, 5, 6, 7, 8, 9, or 10;
• p is 0, 4, 5, 6, 7, 8, 9, or 10;
• A is Lys or Asp;
• B is Lys or Asp;
• n is 1 or 2;
• N is residues 93-131 of SEQ ID NO:30;
• C is residues 142-156 of SEQ ID NO:30;
• Xaa1, Xaa2, Xaa3, Xaa4, Xaa5 are Val, Val, Tyr, Met, and Leu, respectively, in which zero or one to five of residues Xaa1, Xaa2, Xaa3, Xaa4, Xaa5 is substituted with Pro, Asp, Glu, Lys, Gly, or Ser;
• and
• when zero residue is substituted, then either or both of m and p is not zero.

An isolated peptide represented by the formula

• (A)m—(N—Xaa1GlyAlaXaa2Xaa3—C)n—(B)p
• wherein:
• m is 0, 4, 5, 6, 7, 8, 9, or 10;
• p is 0, 4, 5, 6, 7, 8, 9, or 10;
• A is Lys or Asp;
• B is Lys or Asp;
• n is 1 or 2;
• N is residues 1-22 of SEQ ID NO:46;
• C is residues 28-37 of SEQ ID NO:46;
• Xaa1, Xaa2, and Xaa3 are Phe, Ile, and Leu, respectively, in which zero, one, two or three of residues Xaa1, Xaa2, and Xaa3 is substituted with Pro, Asp, Glu, Lys, Gly, or Ser;
• and
• when zero residues is substituted, then either or both m and p is not zero.

An isolated polypeptide or peptide selected from the group consisting of:

• (A) a polypeptide of SEQ ID NO:55 in which one or more of three sets of valine residues, (i) residues 37 and 40, (ii) residues 48, 49, and 52, and (iii) residues 70, 71, and 74, is substituted with all Glu, all Asp, all Pro, all Lys, all Gly, or all Ser residues;
• (B) a peptide consisting of a 30 to 36 residue segment, corresponding to residues 1-30 to 1-36 of SEQ ID NO:54, alone or joined at its N-terminus and/or C-terminus to a polylysine or polyaspartate segment of 4-10 residues in length;
• and
• (C) a peptide consisting of a 30 to 66 residue segment, corresponding to residues 75-140 to 111-140 of SEQ ID NO:54, alone or joined at its N-terminus and/or C-terminus to a polylysine or polyaspartate segment of 4-10 residues in length.