Genes for male accessory gland proteins in Drosophila melanogaster
AssigneeCornell Research Foundation, Inc.
InventorsMariana F. Wolfner, Oliver Lung, Khanh-Uyen Tram
Life of this patent
Estimating the term…
Abstract
The present invention provides a number of accessory gland proteins from Drosophila. The invention also provides an accessory gland protein which is toxic to insect cells and can be used to kill or inhibit the development of insects. Methods of controlling insects are also provided.
Claims45
An isolated nucleic acid molecule encoding an accessory gland protein from Drosophila which has the biological property of an insect toxin.
An isolated Drosophila melanogaster insect toxin protein.
A method of reducing an insect's life span comprising:
- contacting the insect with an isolated Drosophila insect toxin protein under conditions effective to shorten the insect's life span.
A method of reducing an insect's life span comprising:
- contacting the insect with an expression vector containing a nucleic acid molecule encoding a Drosophila insect toxin protein under conditions effective to express the protein.
The isolated nucleic acid molecule wherein the nucleic acid molecule is selected from the group consisting of
- a nucleic acid molecule having a nucleotide sequence selected from the group consisting of SEQ. ID. No. 2, SEQ. ID. No. 5, SEQ. ID. No. 8, SEQ. ID. No. 13, SEQ. ID. No. 18, SEQ. ID. No. 21, SEQ. ID. No. 24, SEQ. ID. No. 27, and SEQ. ID. No. 30;
- and
- a nucleic acid molecule which hybridizes under stringent conditions to a nucleic acid molecule having a nucleic acid sequence selected from the group consisting of SEQ. ID. No. 2, SEQ. ID. No. 5, SEQ. ID. No. 8, SEQ. ID. No. 13, SEQ. ID. No. 18, SEQ. ID. No. 21, SEQ. ID. No. 24, SEQ. ID. No. 27, and SEQ. ID. No. 30.
An isolated protein wherein the protein has an amino acid sequence selected from the group consisting of SEQ. ID. No. 4, SEQ. ID. No. 7, SEQ. ID. No. 11, SEQ. ID. No. 12, SEQ. ID. No. 16, SEQ. ID. No. 17, SEQ. ID. No. 20, SEQ. ID. No. 23, SEQ. ID. No. 26, SEQ. ID. No. 29, and SEQ. ID. No. 32.
A method for determining whether a female Drosophila melanogaster has recently mated comprising:
- providing an antibody, fragment thereof, or probe which recognizes a protein selected from the group consisting of SEQ. ID. No. 4, SEQ. ID. No. 7, SEQ. ID. No. 11, SEQ. ID. No. 12, SEQ. ID. No. 16, SEQ. ID. No. 17, SEQ. ID. No. 20, SEQ. ID. No. 23, SEQ. ID. No. 26, SEQ. ID. No. 29, and SEQ. ID. No. 32, wherein the antibody, fragment thereof, or probe is bound to a label effective to permit detection of said protein upon binding of the antibody, fragment thereof, or probe to said protein;
- contacting a fluid or tissue sample from the female Drosophila melanogaster with the antibody, fragment thereof, or probe having a label under conditions effective to permit binding of the antibody, fragment thereof, or probe to the protein;
- and
- detecting a presence of any of said protein in the biological sample by detecting the label.
Dependencies are inferred from the claim text.