Protein carrier system for therapeutic oligonucleotides
AssigneeMedBridge, Inc.
InventorDong Xie
Life of this patent
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Abstract
Therapeutic oligonucleotides, including antisense oligonucleotides and siRNA, are modified with reactive chemical groups connected by flexible linker molecules. The modified oligonucleotides are capable of forming covalent bonds with mobile proteins, in particular with human serum albumin. While retaining biological activity, the resulting complex has enhanced cellular entry, significantly enhanced serum half-life, and reduced immune system stimulation when compared to unmodified oligonucleotides. The modified oligonucleotides overcome many problems associated with current antisense drugs. Modified oligonucleotides of the invention are administered as therapeutic agents, and hybridize to complementary sequences within targeted RNA molecules.
Claims131
A method of treating a disease by administering a therapeutic oligonucleotide to a patient in need thereof, comprising administering to a patient a therapeutic oligonucleotide including a reactive group, the reactive group upon reaction with a mobile protein forming a covalent bond through which said mobile protein is conjugated to said therapeutic oligonucleotide.
A method of treating a disease by administering a therapeutic oligonucleotide to a patient in need thereof, comprising administering to a patient a therapeutic oligonucleotide conjugated by a covalent bond with a mobile protein.
A method of treating a disease, comprising administering to a patient in need thereof a double-stranded RNA duplex including a reactive group, the reactive group upon reaction with a mobile protein forming a covalent bond through which said mobile protein is conjugated to said double-stranded RNA duplex.
A method of treating a disease, comprising administering to a patient in need thereof a double-stranded RNA duplex conjugated by a covalent bond with a mobile protein.
A therapeutic oligonucleotide of 15-30 bases in length comprising a portion that binds an RNA or DNA target, further including a reactive group bonded to the oligonucleotide, the reactive group upon reaction with a mobile protein forming a covalent bond through which said mobile protein is conjugated to said therapeutic oligonucleotide.
A therapeutic oligonucleotide comprising 15-30 bases in length including a reactive group, the reactive group upon reaction with a mobile protein forming a covalent bond with said mobile protein, wherein the oligonucleotide is selected from the group consisting of SEQ ID NOs:
- 1-22.
A therapeutic oligonucleotide of 15-30 bases in length comprising a portion that binds a RNA or DNA target, wherein said oligonucleotide is conjugated by a covalent bond with a mobile protein.
A therapeutic oligonucleotide comprising a double stranded RNA duplex including a reactive group, the reactive group upon reaction with a mobile protein forming a covalent bond through which said mobile protein is conjugated to said double-stranded RNA duplex.
A therapeutic oligonucleotide comprising a double stranded RNA duplex conjugated by a covalent bond with a mobile protein.
Dependencies are inferred from the claim text.