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US 2003/0166303 A1Published application
Novel screening method for molecular antagonist using flow-cytometry
OwnerDavid Peritt, Jonathan H. Seidemaninventors
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Abstract
The present invention relates to a robust and sensitive assay system utilizing synthetic spherical structures in place of cells for a FACS analysis. The method and system detects the affinity and neutralization activity of a biological molecule without interference from growth medium or cell supernatants. The systems associated with these methods allow high-throughput screening of assays for, in particular, neutralizing monoclonal antibodies.
Claims17
A method for determining the affect of a biological molecule on the binding of two proteins using flow-cytometry comprising:
- combining (i) a first protein-coupled cell-substitute comprising a first protein coupled to a cell-substitute;
- (ii) a second protein that is capable of being labeled with a fluorescent marker, wherein said first and second proteins bind to each other;
- and (iii) titrated amounts of a biological molecule that competes with the second protein for binding with the first protein, or that inhibits the second protein from binding with the first protein, such that unbound protein is removed;
- and
- analyzing the binding of said first protein to said second protein by FACS analysis.
2 more elements
Dependencies are inferred from the claim text.