Multiple binding moiety chromatography device, methods of using and methods of making same

A method of using a chromatography device comprising an artificial membrane support comprising a plurality of species of protein binding moieties non-covalently immobilized thereon, wherein said plurality of species of immobilized protein binding moieties are immobilized such that the tertiary structure of the protein in each immobilized binding moiety permits specific binding to a molecule that is bound by said protein in said immobilized protein binding moiety, said method comprising:

• exposing said artificial membrane support to a liquid flow system.

A support comprising

• (1) at least one species of protein binding moiety immobilized through a covalent bond with the support surface to form an immobilized protein binding moiety, wherein said species of immobilized protein binding moiety is immobilized such that the protein in the immobilized binding moiety permits specific binding to a molecule that is bound by said protein in said immobilized protein binding moiety, and
• (2) at least one marker molecule associated with the protein binding moiety species.

A method of using a chromatography device comprising a support comprising at least one protein binding moiety immobilized through a covalent bond with the support surface to form an immobilized protein binding moiety, wherein said immobilized protein binding moiety is immobilized such that the protein in the immobilized binding moiety permits specific binding to a molecule that is bound by said protein in said immobilized protein binding moiety, said method comprising:

• exposing said support to a liquid flow system containing a marker molecule associated with the protein binding moiety species.

A method for performing drug discovery comprising using a chromatography device having an artificial membrane support comprising a plurality of distinct binding moieties non-covalently immobilized thereon, wherein said plurality of immobilized binding moieties are immobilized such that the tertiary structure of the protein in each immobilized binding moiety permits specific binding to a molecule that is bound by said protein in said immobilized binding moiety, comprising:

• exposing said support to a liquid flow system containing a marker molecule associated with the protein binding moiety species in a process of lead optimization.

A method for performing drug discovery comprising using a chromatography device having a support comprising at least one binding moiety immobilized through a covalent bond with the support surface to form an immobilized binding moiety, wherein said immobilized binding moiety is immobilized such that a protein in the immobilized binding moiety permits specific binding to a molecule that is bound by said protein in said immobilized binding moiety, comprising:

• exposing said support to a liquid flow system containing a marker molecule associated with the protein binding moiety species in a process of lead optimization.

A method of making an artificial membrane support comprising a plurality of distinct binding moieties non-covalently immobilized thereon, wherein said plurality of immobilized binding moieties are immobilized such that the tertiary structure of the protein in each immobilized binding moiety permits specific binding to at least one molecule that is bound by a protein in said plurality of immobilized binding moieties, comprising:

• forming a stationary phase containing a plurality of species of protein having a known binding affinity for a least one marker molecule and combining with a marker molecule associated with the protein binding moiety species.

A method of making a support comprising at least one binding moiety immobilized through a covalent bond with the support surface to form an immobilized binding moiety, wherein said immobilized binding moiety is immobilized such that a protein in the immobilized binding moiety permits specific binding to a molecule that is bound by said protein in said immobilized binding moiety:

• forming a covalent bond linking a protein having a known binding affinity for at least one marker molecule with a surface of the support and combining a marker molecule associated with the protein binding moiety species.