Polynucleotide complex delivery
AssigneeMirus Bio Corporation
InventorsSean D. Monahan, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, David B. Rozema, Paul M. Slattum
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Abstract
Disclosed is a complex for providing nucleic acid expression in a cell. A polynucleotide and a polymer are mixed together to form the complex wherein the zeta potential of the complex is not positive. Then the complex is delivered to the cell wherein the nucleic acid is expressed.
Claims22
A process for delivering a polynucleotide complexed with a compound into an extravascular muscle cell of a mammal, comprising:
- a) mixing the polynucleotide and a polymer to form a complex wherein the zeta potential of the complex is not positive;
- b) inserting the polynucleotide into a mammalian blood vessel, in vivo;
- c) increasing the permeability of the blood vessel;
- d) passing the complex through the blood vessel;
- e) delivering the complex into the mammalian muscle cell;
- and,
- f) expressing the polynucleotide.
The complex of claim 4 wherein the complex is delivered to the cell by increasing the permeability of the vessel;
A complex for in vivo polynucleotide expression, comprising:
- a combination of at least two compounds characterized by the complex zeta potential which is not positive and ability to facilitate delivery of a polynucleotide to a cell where it is expressed.
A process for delivering a polynucleotide complexed with a compound into an extravascular liver cell of a mammal, comprising:
- a) mixing the polynucleotide and a polymer to form a complex wherein the zeta potential of the complex is not positive;
- b) inserting the polynucleotide into a mammalian blood vessel, in vivo;
- c) increasing the permeability of the blood vessel;
- d) passing the complex through the blood vessel;
- e) delivering the complex into the mammalian muscle cell;
- and,
- f) expressing the polynucleotide.
Dependencies are inferred from the claim text.