Cultures of human CNS neural stem cells
OwnerMelissa Carpenterinventor
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Abstract
The invention provides a cell culture including proliferating human neural stem cells with a doubling rate faster than thirty days. The invention also provides a cell culture media for proliferating mammalian neural cells including a standard defined culture medium, a carbohydrate source, a buffer, a source of hormones, one or more growth factors that stimulate the proliferation of neural stem cells, and LIF. The invention also provides a method for protecting, repairing or replacing damaged tissue comprising transplanting mammalian neural stem cells formed into neurospheres. The invention also provides a cell culture of differentiated human neural stem cells where the cells are glioblasts. The invention also provides a method of differentiating human neural stem cells in culture media.
Claims19
A cell culture comprising proliferating human neural stem cells wherein the cells have a doubling rate faster than 30 days.
A cell culture comprising proliferating human neural stem cells wherein the cells have a doubling rate of 5-10 days.
A cell culture comprising human forebrain-derived neural stem cells.
A cell culture comprising differentiated human neural stem cells, and comprising greater than 10% neurons.
A culture media for proliferating mammalian neural stem cells, the media comprising cell viability and cell proliferation effective amounts of the following components:
- (a) a standard defined culture medium;
- (b) a carbohydrate source;
- (c) a buffer;
- (d) a source of hormones;
- (e) one or more growth factors that stimulate proliferation of neural stem cells;
- (f) LIF.
A composition for use in transplantation comprising mammalian neural stem cells, wherein said cells are substantially formed into neurospheres of a diameter between 10-500 μm in diameter.
A method for protecting, repairing or replacing damaged tissue in a patient comprising transplanting mammalian neural stem cells comprising mammalian neural stem cells, wherein said cells are substantially formed into neurospheres of a diameter between 10-500 μm in diameter.
A cell culture comprising differentiated human neural stem cells, wherein said differentiated cells comprise glioblast cells.
A method of differentiating human neural stem cells in culture media, the method comprising:
- (a) removal of the defined growth media containing growth factor mitogens and LIF,
- (b) provision of a substrate onto which the cells can adhere, and
- (c) provision of a defined media, the defined media comprising a standard defined culture media, 1% serum, and a mixture of growth factors comprising of PDGF A/B, CNTF, IGF-1, forskolin, T3, LIF and NT-3.
Dependencies are inferred from the claim text.