Three-dimensional matrix for producing living tissue equivalents

A method of producing a three-dimensional matrix comprising mixing fibroblasts, thrombin, and fibrinogens or a blood plasma to form a mixture.

A method of producing a three-dimensional matrix having a desired shape, comprising:

• obtaining a mold having the desired shape, and
• filing the mold with a mixture of fibroblasts, thrombin, and fibrinogens or a blood plasma.

A living tissue equivalent comprising a fibrin matrix, fibroblasts, and cells, wherein the cells are not fibroblasts.

A method of producing a living tissue equivalent comprising mixing thrombin, fibroblasts, cells, fibrinogens or a blood plasma, wherein the cells are not fibroblasts.

A method of producing a living tissue equivalent having a desired shape, comprising:

• providing a mold having the desired shape;
• and
• filing the mold with a mixture of cells, fibroblasts, thrombin, and fibrinogens or a blood plasma, wherein the cells are not fibroblasts.

A method of screening an agent, comprising:

• mixing a blood plasma or fibrinogens, thrombin, fibroblasts, and cells to form a living tissue equivalent, wherein the cells are not fibroblasts;
• and
• contacting the agent to the living tissue equivalent.

A method of expressing a desired protein in a tissue, comprising:

• transfecting cells with genetic materials to become transfected cells, wherein the transfected cells are capable of expressing the desired protein;
• mixing a blood plasma or fibrinogens, thrombin, fibroblasts, and the transfected cells to form a living tissue equivalent.

A method of producing a bone tissue equivalent, comprising:

• mixing a blood plasma or fibrinogens, thrombin, fibroblasts, and a demineralized bone powder.

A method of producing a bone equivalent, comprising:

• mixing blood plasma, thrombin, fibroblasts, and undifferentiated cells, wherein the undifferentiated cells differentiate to osteoblasts.

A method of producing a cartilage tissue equivalent, comprising:

• mixing blood plasma, thrombin, fibroblasts, and chondrocytes, or chondrocte stem cells.

A tissue microarray comprising a multiplicity of tissue equivalent blocks, wherein the tissue equivalent blocks are formed through slicing a living tissue equivalent comprising a mixture of a blood plasma or fibrinogens, thrombin, fibroblasts, and cells, wherein the cells are not fibroblasts.

A method of replacing a tissue in a body, comprising:

• mixing a blood plasma or fibrinogens, thrombin, fibroblasts, and cells into a living tissue equivalent, wherein the cells are not fibroblasts;
• and
• implementing the living tissue equivalent in the body.