Quality control for cytochemical assays
AssigneeCytoLogix Corporation
InventorsSeshi R. Sompuram, Halasya Ramanathan
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Abstract
The invention describes quality control devices for assays that measure analytes in cells and tissue samples, and methods of use thereof. In particular, the quality control device comprises a matrix affixed with synthetic controls in different concentrations, or different synthetic controls. The quality control device can be adhered to a microscope slide via an adhesive or chemically attached to a microscope slide and processed simultaneously with a tissue sample.
Claims26
A method for attaching a biological molecule to a glass surface comprising the steps of:
- a) providing a silane-treated glass surface having an end-capped amino group;
- b) reacting said end-capped amino group with a phosgene equivalent to form a protected isocyanate group;
- and
- c) reacting said protected isocyanate group with an amino group of a biological molecule, thereby forming a covalent coupling and attaching said biological molecule to the glass surface.
A method for attaching a biological molecule to a glass surface comprising the steps of:
- a) providing a silane-treated glass surface having an end-capped isocyanate group;
- b) reacting said isocyanate group with a blocking reagent thereby forming a protected isocyanate group;
- c) reacting said protected isocyanate group with an amino group of a biological molecule,
- thereby forming a covalent coupling and attaching said biological molecule to the glass surface.
A method for attaching a biological molecule to a glass surface comprising the steps of:
- a) providing a silane-treated glass surface having an end-capped amino group;
- b) reacting said amino group with a phosgene equivalent to form an end-capped group, said end-capped group including a functional group represented by the following structural formula:
- >N—C(O)—N<
- or by the following structural formula:
- >N—C(O)—O—N<;
- and
- c) reacting said end-capped group with an amino group of a biological molecule,
- thereby forming a covalent coupling and attaching said biological molecule to the glass surface.
A method for storing a microscope slide, suitable for covalently attaching a molecule to a surface of said microscope slide, comprising the steps of:
- a) providing a silane-treated microscope slide surface having an end-capped isocyanate group;
- and
- b) reacting said isocyanate group with a blocking reagent thereby forming a protected isocyanate group.
A method for attaching a biological molecule to a silane-treated glass surface comprising the steps of:
- a) reacting an end-capped amino group on said silane-treated glass surface with a phosgene equivalent to form a protected isocyanate group;
- and
- b) reacting said protected isocyanate group with an amino group of a biological molecule,
- thereby forming a covalent coupling and attaching said biological molecule to said silane-treated glass surface.
A method for attaching a biological molecule to a glass-treated surface comprising the steps of:
- a) reacting an end capped isocyanate group on said silane-treated glass surface with a blocking reagent thereby forming a protected isocyanate group;
- b) reacting said protected isocyanate group with an amino group of a biological molecule,
- thereby forming a covalent coupling and attaching said biological molecule to said silane-treated glass surface.
A method for attaching a biological molecule to a silane-treated glass surface comprising the steps of:
- a) reacting an amino group on said silane-treated glass surface with a phosgene equivalent to form an end-capped group, said end-capped group including a functional group represented by the following structural formula:
- >N—C(O)—N<
- or by the following structural formula:
- >N—C(O)—O—N<;
- and
- b) reacting said end-capped group with an amino group of a biological molecule,
- thereby forming a covalent coupling and attaching said biological molecule to said silane-treated glass surface.
A method for storing a microscope slide, suitable for covalently attaching a molecule to a surface of said microscope slide, comprising reacting an isocyanate group on a silane-treated microscope slide surface with a blocking reagent, thereby forming a protected isocyanate group.
Dependencies are inferred from the claim text.