Saccharomyces cerevisiae yeast strain with functional expression of a glut transporter
Assigneesanofi-aventis Pharma Deutschland GmbH
InventorsGunter Muller, Klaus-Peter Koller, Eckhard Boles, Roman Wieczorke, Silke Dlugai
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Abstract
The invention relates to a strain of the yeast Saccharomyces cerevisiae which, owing to deletion of the genomic sequences, no longer synthesizes hexose transporters and, as a consequence, can no longer grow on substrates with hexoses as the only carbon source, and whose ability of growing on a substrate with a hexose as the only carbon source is restored when it expresses a GLUT4 gene.
Claims25
A strain of the yeast Saccharomyces cerevisiae which can no longer grow on substrates with hexoses as the only carbon source, and whose ability of growing on a substrate with a hexose as the only carbon source is restored when a GLUT4 gene is expressed in this strain.
A method which can be used for identifying a compound which increases or reduces the amount of a hexose transported by means of a Glut1 protein, with the following process steps:
- a) Providing a strain of the yeast Saccharomyces cerevisiae which can no longer grow on substrates with hexoses as the only carbon source and whose ability of growing on a substrate with a hexose as the only carbon source is restored when it expresses a Glut1 gene, this strain comprising a GLUT-1 gene under the functional control of a promoter which can be expressed in yeast;
- b) Determining the amount of a hexose which is taken up by this strain provided in accordance with a);
- c) Providing a compound;
- d) Contacting a strain of the yeast provided in accordance with a) with a compound provided in accordance with c);
- e) Determining the amount of a hexose which is taken up into the yeast strain after contacting in accordance with d);
- f) Identifying a compound which increases or reduces the amount of a hexose transported by means of a Glut1 protein by comparing the amount of the hexose taken up into the strain before and after contacting in accordance with d), which is determined in accordance with b) and e).
A strain of the yeast Saccharomyces cerevisiae as deposited at the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH under the Accession Number DSM 14026 or DSM 14027.
A polynucleotide sequence encoding a GLUT1 protein with a substitution of valine with methionine at position 69 of the amino acid sequence.
A polynucleotide sequence encoding a GLUT1 protein with a substitution of valine with methionine at position 70 of the amino acid sequence.
Dependencies are inferred from the claim text.