Cytochrome P450s and uses thereof
AssigneeBoard of Trustees of the University of Kentucky
InventorsJoseph Chappell, Lyle F. Ralston
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Abstract
The invention features isolated cytochrome P450 polypeptides and nucleic acid molecules, as well as expression vectors and transgenic plants containing these molecules. In addition, the invention features uses of such molecules in methods of increasing the level of resistance against a disease caused by a plant pathogen in a transgenic plant, in methods for producing altered compounds, for example, hydroxylated compounds, and in methods of producing isoprenoid compounds.
Claims32
A method for producing an isoprenoid compound, said method comprising the steps of:
- (a) culturing a host cell under conditions suitable for expressing a first recombinant protein comprising an isoprenoid synthase and a second recombinant protein comprising a cytochrome P450 polypeptide, wherein said first and second recombinant protein catalyze the formation of an isoprenoid compound not normally produced by said host cell;
- and
- (b) recovering said isoprenoid compound.
A host cell expressing a recombinant isoprenoid synthase and a recombinant cytochrome P450 polypeptide.
An isolated cytochrome P450 polypeptide having 80% identity to the amino acid sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 3.
An isolated cytochrome P450 polypeptide having 85% identity to the amino acid sequence of SEQ ID NO:
- 5 or SEQ ID NO:
- 7.
An isolated cytochrome P450 polypeptide having 97% identity to the amino acid sequence of SEQ ID NO:
- 11.
An isolated cytochrome P450 nucleic acid molecule which encodes a polypeptide having 80% identity to the amino acid sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 3, or SEQ ID NO:
- 11.
An isolated cytochrome P450 nucleic acid molecule which encodes a polypeptide having 85% identity to the amino acid sequence of SEQ ID NO:
- 5 or SEQ ID NO:
- 7.
An isolated nucleic acid molecule that specifically hybridizes under highly stringent conditions to any one of the complement of the sequence described in SEQ ID NOS:
- 2, 4, 6, 8, or 12, wherein said nucleic acid encodes a cytochrome P450 polypeptide.
Dependencies are inferred from the claim text.