Manipulation of negative stranded RNA viruses by rearrangement of their genes and uses thereof
AssigneeThe UAB Research Foundation
InventorsGail W. Wertz, Andrew L. Ball
Life of this patent
Estimating the term…
Abstract
The present invention provides a method of increasing expression of a promoter distal gene in a virus of the order Mononegavirales, and a recombinant virus constructed by such method. Also provided is a method of attenuating a virus of the order Mononegavirales, and of constructing an attenuated virus useful for a vaccine.
Claims29
A method of increasing expression of a promoter distal gene in a virus of the order Mononegavirales, comprising the step of:
- rearranging gene order of said virus by moving said promoter distal gene toward a wild-type 3′ promoter proximal position site.
A recombinant virus of the order Mononegavirales having a rearranged genome, said genome is rearranged by moving a promoter distal gene of said virus toward a wild-type 3′ promoter proximal position site.
A method of attenuating a virus of the order Mononegavirales, comprising the step of:
- rearranging gene order of said virus by moving a gene away from its wild-type position.
A method of attenuating a virus of the order Mononegavirales, comprising the step of:
- rearranging gene order of said virus by moving a gene away from its wild-type 3′ promoter proximal position site, wherein said gene is an essential limiting factor for genome replication.
A method of constructing an attenuated virus useful for a vaccine, comprising the steps of:
- rearranging gene order of said virus by moving a gene away from its wild-type 3′ promoter proximal position site, wherein said gene is an essential limiting factor for genome replication;
- and
- placing a gene coding for an immune response-inducing antigen in the position closest to the 3′end of the gene order of said virus, therefore, an attenuated virus is constructed for vaccine use.
A virus attenuated according to the method of claim
Dependencies are inferred from the claim text.