Isolated genomic polynucleotide fragments that encode human lipoprotein-associated phospholipase A2
AssigneeRyogen LLC
InventorJames W. Ryan
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Abstract
The invention is directed to isolated genomic polynucleotide fragments that encode human lipoprotein-associated phospholipase A2, vectors and hosts containing the fragment and fragments hybridizing to noncoding regions as well as antisense oligonucleotides to these fragments. The invention is further directed to methods of using these fragments to obtain human lipoprotein-associated phospholipase A2 and to diagnose, treat, prevent and/or ameliorate a pathological disorder.
Claims22
An isolated genomic polynucleotide, said polynucleotide obtainable from human genomic clone library RP11-446F17 having a nucleotide sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) a polynucleotide encoding human lipoprotein-associated phospholipase A2 depicted in SEQ ID NO:1;
- (b) a polynucleotide consisting of SEQ ID NO:2 which encodes human lipoprotein-associated phospholipase A2 depicted in SEQ ID NO:1
- (c) a polynucleotide which ia a variant of SEQ ID NO:2;
- (d) a polynucleotide which is an allelic variant of SEQ ID NO:2;
- (e) a polynucleotide which encodes a variant of SEQ ID NO:1;
- a polynucleotide which hybridizes to any one of the polynucleotides specified in (a)-(e)
- a polynucleotide that is a reverse complement of the polynucleotides specified in (a)-(f) and containing at least 10 transcription factor binding sites selected from the group consisting of AP1-C, AP1_Q4, AP4-Q5, DELTAEF1-01, GATA1—04, GATA1-06, GATA2-02, GATA3—02, GATA-C, LMO2COM-02, LYF1-01, MYOD_Q6, MZF—01, NFAT_Q6, NKX25-01, S8-01, SOX5-01, TATA-C, and TCF11-01
- as well as nucleic acid constructs, expression vectors and host cells containing these polynucleotide sequences.
An isolated polynucleotide which hybridizes to a non-coding region of SEQ ID NO:2, which non-coding region is selected from the group consisting of an intron, a splice junction, a 5′- non-coding region, a transcription factor binding region and a 3′- non-coding region.
11 A composition comprising the polynucleotide of claim 7 and a carrier.
The kit according to claim 17, in which the antisense oligonucleotide is labeled with a detectable substance.
An isolated polynucleotide which hybridizes to a transcriptional regulatory region of SEQ ID NO:2
Dependencies are inferred from the claim text.