Alpha1,3-fucosyltransferase
AssigneeThe Governors of the University of Alberta, a Canadian corporation
InventorsDiane E. Taylor, Zhongming Ge
Life of this patent
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Abstract
A bacterial α1,3-fucosyltransferase gene and deduced amino acid sequence is provided. The gene is useful for preparing α1,3-fucosyltransferase polypeptide, and active fragment thereof, which can be used in the production of oligosaccharides such as Lewis X, Lewis Y, and siayl Lewis X, which are structurally similar to certain tumor-associated carbohydrate antigens found in mammals. These product glycoconjugates also have research and diagnostic utility in the development of assays to detect mammalian tumors. In addition the polypeptide of the invention can be used to develop diagnostic and research assays to determine the presence of H. pylori in human specimens.
Claims20
An isolated polynucleotide encoding a substantially purified transmembrane segment-free polypeptide having α1,3-fucosyltransferase activity, or enzymatically active portions thereof, and its complement.
A polynucleotide selected from the group consisting of:
- a) SEQ ID NO:4;
- b) SEQ ID NO:4, wherein T is U:
- c) nucleic acid sequences complementary to a) or b);
- and
- d) fragments of a), b), or c) that are at least 15 nucleotide bases in length and that hybridize to DNA which encodes any one of the polypeptide set forth in SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3.
Dependencies are inferred from the claim text.