Alpha-1,3 fucosyltransferases and expression systems for making and expressing them
AssigneeThe Governors of the University of Alberta, a Canadian corporation
InventorsDiane E. Taylor, Zhongming Ge
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Abstract
A bacterial α1,3-fucosyltransferase gene and deduced amino acid sequence is provided. The gene is useful for preparing α1,3-fucosyltransferase polypeptide, and active fragment thereof, which can be used in the production of oligosaccharides such as Lewis X, Lewis Y, and siayl Lewis X, which are structurally similar to certain tumor-associated carbohydrate antigens found in mammals. These product glycoconjugates also have research and diagnostic utility in the development of assays to detect mammalian tumors. In addition the polypeptide of the invention can be used to develop diagnostic and research assays to determine the presence of H. pylori in human specimens.
Claims9
A substantially purified transmembrane segment-free α1,3-fucosyltransferase polypeptide.
A substantially purified transmembrane segment-free α1,3-fucosyltransferase comprising a polypeptide having at least one repeat of the sequence comprising X1X2LRX3X4Y, wherein X1 is D or N;
- X2 is D or N;
- X3 is I, V or A;
- X4 is N or D.
Dependencies are inferred from the claim text.