Process for stabilizing oxidase, stabilized oxidase, mutant oxidase and DNA
AssigneeKabushiki Kaisha Toyota Chuo Kenkyusho
InventorsHaruo Takahashi, Chie Imamura
Life of this patent
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Abstract
The surface of oxidase is stabilized by stabilizing the inner structure responsible for the enzyme function of the oxidase via the alteration of unstable amino acids therein into stable amino acids, and/or immobilizing the oxidase in a structure unit with structural stability and of a given inner diameter. The inner structure of oxidase is stabilized by altering specific unstable amino acids positioned within a 12-angstrom radius range from the active center of the oxidase or altering specific unstable amino acids facing the hydrogen peroxide-binding pocket of the oxidase. The process enhances the stability of the oxidase, particularly the stability of oxidase with lignin degradation activity against hydrogen peroxide.
Claims17
A process for stabilizing oxidase, comprising
- an inner structure stabilization step of stabilizing an inner structure responsible for oxidase function, and
- a surface stabilization step of stabilizing oxidase surface.
A mutant oxidase, which is prepared by partially or wholly altering oxidizable amino acids or amino acids with steric configurations readily modifiable, as positioned within a radius range of 12 angstroms from the active center in the active-type steric configuration of oxidase, into non-oxidizable amino acids or amino acids with steric configurations hardly modifiable.
A mutant oxidase, which is prepared by altering at least one oxidizable amino acid or amino acid with a steric configuration readily modifiable among amino acids facing a hydrogen peroxide-binding pocket of oxidase, into at least one non-oxidizable amino acid or amino acid with a steric configuration hardly modifiable.
Dependencies are inferred from the claim text.