Process for clavulanic acid production
AssigneeSmithKline Beecham Corporation
InventorsCecilia Anders, Barry Barton, John Patrick Griffin, Susan Jensen, Roy Henrey Mosher, Ashish Sudhakar Paradkar
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Abstract
Novel bacterial genes, microorganisms and processes for improving the manufacture of 5R clavams, eg. clavulanic acid.
Claims35
DNA comprising one or more genes specific for 5S clavam biosynthesis in S. clavuligerus and which is not essential for 5R clavam biosynthesis.
S. clavuligerus comprising DNA corresponding to an open reading frame flanking cas1 which DNA has been disrupted or otherwise made defective.
A process for improving 5R clavam production in a suitable microorganism comprising manipulation of DNA as defined in any 1 and its inclusion in the said microorganism.
A process for improving 5R clavam production in S. clavuligerus comprising disrupting or otherwise making defective DNA regions flanking cas1.
A process for the identification of a microorganism suitable for high 5R clavam production comprising a preliminary screening for microorganisms with low or no 5S clavam production.
Clavulanic acid which is free of any 5S clavam.
Clavulanic acid which is free of any clavam-2-carboxylate.
Dependencies are inferred from the claim text.