Adenoviral library assay for E2F regulatory genes and methods and compositions for screening compounds
OwnerHelmuth van Es, Rene Bernards +3inventors
InventorsHelmuth van Es, Rene Bernards, Godefridus A.M. Michiels, Reginald C.X. Brys, Peter H. M. Tomme
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Abstract
Abstract The invention relates to the field of molecular genetics and medicine. In particular the present invention relates to the field of functional genomics. The present invention provides the methods and means for the identification of nucleic acids and the polypeptides encoded by these nucleic acids that have a function related to the E2F pathway, which were isolated in a high-throughput screening assay using the E2F transcription factor activity as a read-out. The identified compounds are suitable drug-targets to treat human diseases.
Claims44
An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:
- a) a polypeptide comprising an amino acid sequence of SEQ ID NO:
- 14,
- b) a naturally occurring polypeptide comprising an amino acid sequence at least 90% identical to an amino acid sequence of SEQ ID NO:
- 14,
- c) a biologically active fragment of a polypeptide having an amino acid sequence of SEQ ID NO:
- 14, and
- d) an immunogenic fragment of a polypeptide having an amino acid sequence of SEQ ID NO:
- 14.
An isolated polynucleotide comprising a sequence selected from the group consisting of:
- a) a polynucleotide comprising a polynucleotide sequence of SEQ ID NO:
- 13,
- b) a naturally occurring polynucleotide comprising a polynucleotide sequence at least 90% identical to a polynucleotide sequence of SEQ ID NO:
- 13,
- c) a polynucleotide having a sequence complementary to a polynucleotide of a),
- d) a polynucleotide having a sequence complementary to a polynucleotide of b) and
- e) an RNA equivalent of a)-d).
Dependencies are inferred from the claim text.