Method for production of hydroxylated collagen-like compounds
OwnerEric Christiaan De Bruin, Marc Willem Theodoor Werten +1inventors
InventorsEric Christiaan De Bruin, Marc Willem Theodoor Werten, Frederik Anton De Wolf
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Abstract
The present invention relates to a method for the production of collagen-like compounds containing hydroxylated proline residues. Of specific interest is the production of recombinant collagen-like compounds in which hydroxylation of proline residues is achieved by a prolyl hydroxylase from a fungus, preferably a yeast, in particular Hansenula polymorpha. Also the invention concerns a method for controlling the hydroxylation of proline residues by such a prolyl hydroxylase characterised by the addition of collagen-like oligopeptides, such as gelatine hydrolysate, in particular gelatone or peptone.
Claims18
Method for the production of collagen-like compounds containing hydroxylated proline residues characterised by using a fungal prolyl hydroxylase.
Method according to any of the preceding claims in which recombinant collagen-like compounds are produced.
Method for the production of endogenous fungal collagen-like compounds comprising the steps of culturing a fungus or fungus-like eukaryotic microorganism, and isolating the endogenous fungal collagen-like compound.
Method according to any of the preceding claims in which the hydroxylation of proline residues by the fungal prolyl hydroxylase is controlled by the addition of a collagen-like oligopeptide.
Collagen-like compound obtainable according to any of the preceding claims.
Composition comprising a prolyl hydroxylase from a fungus, preferably from Hansenula polymorpha.
Prolyl hydroxylase from a fungus, preferably from Hansenula polymorpha.
Dependencies are inferred from the claim text.