Clonal myeloma cell lines useful for manufacturing proteins in chemically defined media
OwnerChiChang Lee, Edward Savino +2inventors
InventorsChiChang Lee, Edward Savino, Gordon Moore, Celia Ly
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Abstract
The present invention relates to clonal myeloma cell lines that have the ability to grow continuously in chemically defined media. The present invention also relates to the production of proteins in clonal myeloma cell lines and any cell lines derived therefrom. The present invention further relates to methods for identifying cell lines capable of growing in chemically defined media. The present invention also relates to business methods where customers are provided with the cells, cell lines, and cell cultures of the present invention.
Claims34
A clonal myeloma cell line or any cell line derived capable of:
- growing continuously in a chemically defined medium;
- growing to high cell density in a chemically defined medium;
- remaining viable after cryopreservation in the absence of serum;
- and
- detectably expressing recombinant proteins following genetic manipulation and/or subsequent culture in a chemically defined medium.
A method for identifying cell lines capable of growing continuously in a chemically defined medium comprising the steps of:
- culturing cells from one type of cell line in at least one type of chemically defined medium, wherein said cultured cells from one type of cell line are not known to grow in said chemically defined medium;
- and
- selecting spontaneous mutant cells that are capable of growing in said chemically defined medium.
Dependencies are inferred from the claim text.