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US 2003/0166141 A1Published application
Regulation of endogenous gene expression in cells using zinc finger proteins
AssigneeSangamo BioSciences, Inc.
InventorsCasey C. Case, George N. Cox III, Stephen P. Eisenberg, Qiang Liu, Edward J. Rebar
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Abstract
The present invention provides methods for modulating expression of endogenous cellular genes using engineered zinc finger proteins.
Claims34
A method for modulating expression of an endogenous gene in a cell, wherein the method comprises:
- a) administering to the cell a nucleic acid molecule comprising a polynucleotide sequence encoding an engineered zinc finger protein, wherein the engineered zinc finger protein comprises a plurality of zinc fingers and further wherein the amino acid sequence of at least one of the zinc fingers is obtained from the information in a database of existing zinc finger proteins and their respective binding affinities;
- and
- b) maintaining the cell under conditions in which the engineered zinc finger protein is expressed and binds to a target site in the gene.
A method for modulating expression of an endogenous gene in a cell, wherein the method comprises:
- a) administering to the cell a nucleic acid molecule comprising a polynucleotide sequence encoding an engineered zinc finger protein, wherein the engineered zinc finger protein comprises a plurality of zinc fingers and further wherein each zinc finger is obtained from a preexisting zinc finger protein;
- and
- b) maintaining the cell under conditions in which the engineered zinc finger protein is expressed and binds to a target site in the gene.
A method for modulating expression of an endogenous gene in a cell, wherein the method comprises:
- a) administering to the cell a nucleic acid molecule comprising a polynucleotide sequence encoding an engineered zinc finger protein, wherein the zinc finger protein comprises a plurality of zinc fingers and further wherein the zinc finger protein is partially randomized;
- and
- b) maintaining the cell under conditions in which the engineered zinc finger protein is expressed and binds to a target site in the gene.
Dependencies are inferred from the claim text.