Mammalian sweet and amino acid heterodimeric taste receptors
AssigneeThe Regents of the University of California
InventorsCharles S. Zuker, Jayaram Chandrashekar, Greg Nelson, Yifeng Zhang, Nicholas J.P. Ryba, Mark A. Hoon
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Abstract
The present invention provides isolated nucleic acid and amino acid sequences of sweet or amino acid taste receptors comprising two heterologous G-protein coupled receptor polypeptides from the T1R family of sensory G-protein coupled receptors, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of sweet and amino acid taste receptors.
Claims81
An isolated taste receptor comprising a T1R3 polypeptide, wherein the T1R3 polypeptide is encoded by a nucleotide sequence that hybridizes under moderately stringent hybridization conditions to a nucleotide sequence encoding an amino acid sequence of SEQ ID NO:15, 20, 23, 25, or 30.
An isolated taste receptor comprising a T1R3 polypeptide and a T1R1 polypeptide, wherein the T1R3 polypeptide is encoded by a nucleotide sequence that hybridizes under highly stringent hybridization conditions to a nucleotide sequence encoding an amino acid sequence of SEQ ID NO:15, 20, 23, 25 or 30;
- and wherein the T1R1 polypeptide that is encoded by a nucleotide sequence that hybridizes under moderately stringent hybridization conditions to a nucleotide sequence encoding an amino acid sequence of SEQ ID NO:1, 2, 3, or 27.
An isolated taste receptor comprising a T1R3 polypeptide and a T1R2 polypeptide, wherein the T1R3 polypeptide is encoded by a nucleotide sequence that hybridizes under highly stringent hybridization conditions to a nucleotide sequence encoding an amino acid sequence of SEQ ID NO:15, 20, 23, 25 or 30;
- and wherein the T1R2 polypeptide that is encoded by a nucleotide sequence that hybridizes under moderately stringent hybridization conditions to a nucleotide sequence encoding an amino acid sequence of SEQ ID NO:7, 8, or 9.
A method of identifying a compound that modulates taste signal transduction in taste cells, the method comprising the steps of
- (i) contacting the compound with a taste receptor comprising a T1R3 polypeptide, wherein the T1R3 polypeptide is encoded by a nucleotide sequence that hybridizes under moderately stringent hybridization conditions to a nucleotide sequence encoding an amino acid sequence of SEQ ID NO:15, 20, 23, 25 or 30;
- and
- (ii) determining the functional effect of the compound upon the receptor, thereby identifying a compound that modulates taste signal transduction.
A method of identifying a compound that modulates taste signal transduction in taste cells, the method comprising the steps of
- (i) contacting the compound with cell expressing a taste receptor comprising a T1R3 polypeptide and a T1R2 polypeptide, wherein the T1R3 polypeptide is encoded by a nucleotide sequence that hybridizes under highly stringent hybridization conditions to a nucleotide sequence encoding an amino acid sequence of SEQ ID NO:15, 20, 23, 25, or 30;
- and wherein the T1R2 polypeptide that is encoded by a nucleotide sequence that hybridizes under moderately stringent hybridization conditions to a nucleotide sequence encoding an amino acid sequence of SEQ ID NO:7, 8, or 9;
- and
- (ii) determining the functional effect of the compound upon the receptor, thereby identifying a compound that modulates taste signal transduction.
A method of identifying a compound that modulates taste signal transduction in taste cells, the method comprising the steps of
- (i) contacting the compound with cell expressing a taste receptor comprising a T1R3 polypeptide and a T1R1 polypeptide, wherein the T1R3 polypeptide is encoded by a nucleotide sequence that hybridizes under highly stringent hybridization conditions to a nucleotide sequence encoding an amino acid sequence of SEQ ID NO:15, 20, 23, 25, or 30;
- and wherein the T1R1 polypeptide that is encoded by a nucleotide sequence that hybridizes under moderately stringent hybridization conditions to a nucleotide sequence encoding an amino acid sequence of SEQ ID NO:1, 2, 3, or 27;
- and
- (ii) determining the functional effect of the compound upon the receptor, thereby identifying a compound that modulates taste signal transduction.
An isolated nucleic acid that hybridizes under moderately stringent conditions to a nucleic acid that encodes an amino acid sequence of SEQ ID NO:
- 15, 20, 23, 25, or 30.
An isolated polypeptide encoded by a nucleic acid that hybridizes under moderately stringent conditions to a nucleic acid that encodes an amino acid sequence of SEQ ID NO:
- 15, 20, 23, 25, or 30.
Dependencies are inferred from the claim text.