Nucleotide sequences for the control of the expression of DNA sequences in a cell host
AssigneeInstitut Pasteur
InventorsDidier Lereclus, Herve Agaisse
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Abstract
The present invention provides nucleotide sequences from Bacillus bacteria, which control the expression of other DNA sequences in a cell host.
Claims43
DNA sequence for the control of the expression of a sequence coding for a gene in a host cell, said sequence being characterized in that it comprises a promoter and a nucleotide sequence or downstream region under the control of said promoter, and in particular situated downstream from said promoter and upstream from said coding sequence, said nucleotide sequence or downstream region containing a region essentially complementary to the 3′ end of a bacterial ribosomal RNA, said DNA sequence being capable of intervening in order to increase the expression of said coding sequence, placed downstream in a cell host.
DNA sequence according to claims 1 to 18, characterized by:
- its capacity to hybridize under non-stringent conditions with a nucleotide sequence selected from the sequences Seq No.1, Seq. No.2 and Seq. No.3 and Seq. No.4 and by
- its capacity to intervene in the control of the expression in a cell host, in particular of the Bacillus type, of a coding nucleotide sequence placed downstream.
Nucleotide sequence characterized in that it contains a region essentially complementary to the 3′ end of the 16S RNA of a bacterial ribosome, said sequence being capable of intervening at the post-transcriptional level when the sequencing coding for a gene is being expressed, in particular without acting directly on the translation of the coding sequence to be expressed, said nucleotide sequence being under the control of a promoter controlling said coding sequence.
Cell host, characterized in that it is the strain 407-OA::KmR (pHT305D) deposited with the CNCM on May 3, 1994 under No. I-1412.
Dependencies are inferred from the claim text.