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US 2003/0166069 A1Published application
Interleukin-1 receptor antagonist-like molecules and uses thereof
AssigneeAmgen Inc.
InventorsAndrew A. Welcher, Roland Luethy, Shuqian Jing
Life of this patent
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Abstract
The present invention provides novel Interleukin-1 Receptor Antagonist-Like (IL-1ra-L) polypeptides and nucleic acid molecules encoding the same. The invention also provides selective binding agents, vectors, host cells, and methods for producing IL-1ra-L polypeptides. The invention further provides pharmaceutical compositions and methods for the diagnosis, treatment, amelioration, and/or prevention of diseases, disorders, and conditions associated with IL-1ra-L polypeptides.
Claims19
An isolated polypeptide comprising:
- (a) the amino acid sequence as set forth in SEQ ID NO:
- 2;
- or
- (b) the amino acid sequence encoded by the DNA insert in ATCC Deposit No. PTA-1215.
1 more element
An isolated polypeptide comprising:
- (a) an amino acid sequence for an ortholog of SEQ ID NO:
- 2;
- (b) an amino acid sequence that is at least about 70 percent identical to the amino acid sequence of SEQ ID NO:
- 2, wherein the isolated polypeptide has an activity of a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2;
- (c) a fragment of the amino acid sequence set forth in SEQ ID NO:
- 2 comprising at least about 25 amino acid residues, wherein the isolated polypeptide has an activity of a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2, or is antigenic;
- or
- (d) an amino acid sequence for an allelic variant or splice variant of the amino acid sequence as set forth in SEQ ID NO:
- 2, the amino acid sequence encoded by the DNA insert in ATCC Deposit No. PTA-1215, or the amino acid sequence of (a) or (b).
8 more elements
An isolated polypeptide comprising the amino acid sequence as set forth in SEQ ID NO:
- 2 with at least one modification that is an amino acid substitution, an amino acid insertion, an amino acid deletion, C-terminal truncation, or N-terminal truncation, wherein the isolated polypeptide has an activity of a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2.
An isolated polypeptide encoded by a nucleic acid molecule comprising:
- (a) the nucleotide sequence as set forth in SEQ ID NO:
- 1;
- (b) the nucleotide sequence of the DNA insert in ATCC Deposit No. PTA-1215;
- (c) a nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2;
- or
- (d) a nucleotide sequence that hybridizes under at least moderately stringent conditions to the complement of the nucleotide sequence of any of (a)-(c);
- wherein the isolated polypeptide has an activity of a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2.
6 more elements
An isolated polypeptide encoded by a nucleic acid molecule comprising:
- (a) a nucleotide sequence encoding a polypeptide that is at least about 70 percent identical to a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2;
- (b) a nucleotide sequence encoding an allelic variant or splice variant of the nucleotide sequence as set forth in SEQ ID NO:
- 1, the nucleotide sequence of the DNA insert in ATCC Deposit No. PTA-1215, or the nucleotide sequence of (a);
- (c) a region of the nucleotide sequence of SEQ ID NO:
- 1, the DNA insert in ATCC Deposit No. PTA-1215, or the nucleotide sequence of (a) or (b), encoding a polypeptide fragment of at least about 25 amino acid residues;
- or
- (d) a nucleotide sequence that hybridizes under at least moderately stringent conditions to the complement of the nucleotide sequence any of (a)-(c);
- wherein the isolated polypeptide has an activity of a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2.
7 more elements
An isolated polypeptide encoded by a nucleic acid molecule comprising:
- (a) a nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2 with at least one conservative amino acid substitution;
- (b) a nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2 with at least one amino acid insertion;
- (c) a nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2 with at least one amino acid deletion;
- (d) a nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2 that has a C- and/or N-terminal truncation;
- (e) a nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2 with at least one modification that is an amino acid substitution, an amino acid insertion, an amino acid deletion, C-terminal truncation, or N-terminal truncation;
- or
- (f) a nucleotide sequence that hybridizes under at least moderately stringent conditions to the complement of the nucleotide sequence any of (a)-(e);
- wherein the isolated polypeptide has an activity of a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2.
11 more elements
A polypeptide produced by a process comprising:
- (a) culturing a host cell containing a vector comprising a nucleic acid molecule having:
- (i) the nucleotide sequence as set forth in SEQ ID NO:
- 1;
- (ii) the nucleotide sequence of the DNA insert in ATCC Deposit No. PTA-1215;
- (iii) a nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2;
- or
- (iv) a nucleotide sequence that hybridizes under at least moderately stringent conditions to the complement of the nucleotide sequence of any of (i)-(iii);
- under conditions suitable to express the polypeptide;
- and optionally
- (b) isolating the polypeptide from the culture.
8 more elements
A polypeptide produced by a process comprising:
- (a) culturing a host cell containing a vector comprising a nucleic acid molecule having:
- (i) a nucleotide sequence encoding a polypeptide that is at least about 70 percent identical to a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2, wherein the encoded polypeptide has an activity of the polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2;
- (ii) a nucleotide sequence encoding an allelic variant or splice variant of the nucleotide sequence set forth in SEQ ID NO:
- 1, the nucleotide sequence of the DNA insert in ATCC Deposit No. PTA-1215, or the nucleotide sequence of (i);
- (iii) a region of the nucleotide sequence of SEQ ID NO:
- 1, the DNA insert in ATCC Deposit No. PTA-1215, or the nucleotide sequence of (i) or (ii), encoding a polypeptide fragment of at least about 25 amino acid residues, wherein the encoded polypeptide has an activity of a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2, or is antigenic;
- or
- (iv) a nucleotide sequence that hybridizes under at least moderately stringent conditions to the complement of the nucleotide sequence of any of (i)-(iii);
- under conditions suitable to express the polypeptide;
- and optionally
- (b) isolating the polypeptide from the culture.
11 more elements
A polypeptide produced by a process comprising:
- (a) culturing a host cell containing a vector comprising a nucleic acid molecule comprising:
- (i) a nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2 with at least one conservative amino acid substitution, wherein the encoded polypeptide has an activity of the polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2;
- (ii) a nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2 with at least one amino acid insertion, wherein the encoded polypeptide has an activity of the polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2;
- (iii) a nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2 with at least one amino acid deletion, wherein the encoded polypeptide has an activity of the polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2;
- (iv) a nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2 that has a C- and/or N-terminal truncation, wherein the encoded polypeptide has an activity of the polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2;
- (v) a nucleotide sequence encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2 with at least one modification that is an amino acid substitution, an amino acid insertion, an amino acid deletion, C-terminal truncation, or N-terminal truncation, wherein the encoded polypeptide has an activity of the polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2;
- (vi) a nucleotide sequence that hybridizes under at least moderately stringent conditions to the complement of the nucleotide sequence of any of (i)-(v);
- under conditions suitable to express the polypeptide;
- and optionally
- (b) isolating the polypeptide from the culture.
17 more elements
Dependencies are inferred from the claim text.