Peptidoglycan recognition proteins and their production
OwnerMasaaki Ashida, Masanori Ochiai +1inventors
InventorsMasaaki Ashida, Masanori Ochiai, Masakazu Tsuchiya
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Abstract
A gene coding for a peptidoglycan recognition peptide (PGRP) is cloned, a recombinant vector into which said gene is introduced is obtained, and a transformant transformed with said recombinant vector is cultivated, thereby producing the PGRP in large amounts at high purity.
Claims13
A recombinant protein comprising the following amino acid sequence (SEQ ID NO:
- 1):
- 12 Asp Cys Asp Val Val Ser Lys Lys Gln Trp Asp Gly Leu Ile Pro Val His Val Ser Tyr Leu Ala Arg Pro Val Ser Leu Val Ile Val Gln His Thr Val Thr Pro Phe Cys Arg Thr Asp Ala Gly Cys Glu Glu Leu Val Arg Asn Ile Gln Thr Asn His Met Glu Ala Leu Gln Tyr Trp Asp Ile Gly Pro Ser Phe Leu Val Gly Gly Asn Gly Lys Val Tyr Glu Gly Ser Gly Trp Leu His Val Gly Ala His Thr Tyr Gly Tyr Asn Ser Arg Ser Ile Gly Val Ala Phe Ile Gly Asn Phe Asn Thr Asp Glu Pro Ser Gly Ala Met Leu Glu Ala Leu Arg Ser Leu Leu Arg Cys Gly Val Glu Arg Gly His Leu Ala Gly Asp Tyr Arg Val Val Ala His Arg Gln Leu Ile Ala Ser Glu Ser Pro Gly Arg Lys Leu Tyr Asn Gln Ile Arg Arg Trp Pro Glu Trp Leu Glu Asn Val Asp Ser Ile Lys Asn Ala.
A gene coding for a protein comprising the following amino acid sequence (SEQ ID NO:
- 1):
- 14 Asp Cys Asp Val Val Ser Lys Lys Gln Trp Asp Gly Leu Ile Pro Val His Val Ser Tyr Leu Ala Arg Pro Val Ser Leu Val Ile Val Gln His Thr Val Thr Pro Phe Cys Arg Thr Asp Ala Gly Cys Glu Glu Leu Val Arg Asn Ile Gln Thr Asn His Met Glu Ala Leu Gln Tyr Trp Asp Ile Gly Pro Ser Phe Leu Val Gly Gly Asn Gly Lys Val Tyr Glu Gly Ser Gly Trp Leu His Val Gly Ala His Thr Tyr Gly Tyr Asn Ser Arg Ser Ile Gly Val Ala Phe Ile Gly Asn Phe Asn Thr Asp Glu Pro Ser Gly Ala Met Leu Glu Ala Leu Arg Ser Leu Leu Arg Cys Gly Val Glu Arg Gly His Leu Ala Gly Asp Tyr Arg Val Val Ala His Arg Gln Leu Ile Ala Ser Glu Ser Pro Gly Arg Lys Leu Tyr Asn Gln Ile Arg Arg Trp Pro Glu Trp Leu Glu Asn Val Asp Ser Ile Lys Asn Ala.
A gene coding for a protein comprising the following amino acid sequence (SEQ ID NO:
- 2)
- 16 Met Ala Arg Leu His Ser Ala Val Val Leu Ala Leu Ala Leu Ser Ser Leu Leu Thr Glu Ile Ala Ala Asp Cys Asp Val Val Ser Lys Lys Gln Trp Asp Gly Leu Ile Pro Val His Val Ser Tyr Leu Ala Arg Pro Val Ser Leu Val Ile Val Gln His Thr Val Thr Pro Phe Cys Axg Thr Asp Ala Gly Cys Glu Glu Leu Val Arg Asn Ile Gin Thr Asn His Met Glu Ala Leu Gln Tyr Trp Asp Ile Gly Pro Ser Phe Leu Val Gly Gly Asn Gly Lys Val Tyr Glu Gly Ser Gly Trp Leu His Val Gly Ala His Thr Tyr Gly Tyr Asn Ser Arg Ser Ile Gly Val Ala Phe Ile Gly Asn Phe Asn Thr Asp Glu Pro Ser Gly Ala Met Leu Glu Ala Leu Arg Ser Leu Leu Arg Cys Gly Val Glu Arg Gly His Leu Ala Gly Asp Tyr Arg Val Val Ala His Arg Gln Leu Ile Ala Ser Glu Ser Pro Gly Arg Lys Leu Tyr Asn Gin Ile Arg Arg Trp Pro Glu Trp Leu Glu Asn Val Asp Ser Ile Lys Asn Ala.
A gene comprising a DNA having the following nucleotide sequence (SEQ ID NO:
- 3):
- 18 GATTGCGACGTCGTCAGTAAAAAGCAATGGGACGGTTTGATCCCGGTGCA CGTGTCGTACCTGGCGCGGCCCGTGAGCCTCGTCATCGTCCAGCACACAG TCACACCCTTCTGCAGGACGGACGCTGGCTGCGAGGAGCTCGTGCGTATC CAGACCAACCACATGGAGGCCTTGCAATACTGGGACATCGGACCCTCGTT CCTGGTGGGAGGTAACGGCAAGGTGTACGAGCGCTCCGGCTGCCTGCACG TCGGCGCGCACACCTACGGGTACAACTCGACGTCCATCGGAGTCCATTCA TCGGCAACTTCAACACGCACGACCCGAGCGGCGCGATGCTGGAGGCGCTG CGGTCGCTGCTGCGCTGCGGCGTGGAGCGCGGCCACCTCGCGGGGGACTA CCGCGTCGTGCCGCACCGACAGCTCATTGCCTCTGAGAGCCCCGGCCCGG AACTCTACAACCAGATACGACGCTGGCCTGAGTGGCTGGAGAACGTGGAC TCCATCAAGAACGCGTAA.
A gene comprising a DNA including a nucleotide sequence which codes a protein having peptidoglycan recognition activity and is capable of hybridizing with the DNA having the following nucleotide sequence (SEQ ID NO:
- 3) under stringent conditions:
- 19 GATTGCGACGTCGTCAGTAAAAAGCAATGGGACGGTTTGATCCCGGTGCA CGTGTCGTACCTGGCGCGGCCCGTGAGCCTCGTCATCGTCCAGCACACAG TCACACCCTTCTGCAGGACGGACGCTGGCTGCGAGGAGCTCGTGCGGAAT ATCCAGACCAACCACATGGAGGCCTTGCAATACTGGGACATCGGACCCTC GTTCCTGGTGGGAGGTAACGGCAAGGTGTACGAGGGCTCCGGCTGGCTGC ACGTCGGCGCGCACACCTACGGGTACAACTCGAGGTCCATCGGAGTCGCA TTCATCGGCAACTTCAACACGGACGGCCGAGCGGCGCGATGCTGGAGGCG CTGCGGTCGCTGCTGCGCTGCGGCGTGGAGCGCGGCCACCTCGCGGGGGA CTACCGCGTCGTGGCGCACCGACAGCTCATTGCCTCTGAGAGCCCCGGCC GGAAGCTCTACAAACCAGATACGACGCTGGCCTGAGTGGCTGGAGAACGT GGACTCCATCAAGAACGCGTAA.
A gene comprising a DNA having the following nucleotide sequence (SEQ ID NO:
- 5):
- 20 ATGGCCCGCCTCCACTCGGCAGTTGTACTCGCGCTCGCTCTCAGCTCGCT TCTCACAGAAATAGCAGCCGATTGCGACGTCGTCAGTAAAAAGCAATGGG ACGGTTTGATCCCGGTGCACGTGTCGTACCTGGCGCGGCCCGTGAGCCTC GTCATCGTCCAGCACACAGTCACACCCTTCTGCAGGACGGACGCTGGCTG CGAGCAGCTCGTGCGAATATCCAGACCAACCACATGGAGGCCTTGCAATA CTGGGACATCGGACCCTCGTTCCTGGTGGGAGGTAACGGCAAGGTGTACG AGGGCTCCGGCTGGCTGCACGTCGGCGCGCACACCTACGGGTACAACTCG AGGTCCATCGGAGTCGCATTCATCGGCAACTTCAACACGGACGAGCCGAG CGGCGCGATGCTGGAGGCGCTGCGGTCGCTGCTGCGCTGCGGCGTGGAGC GCGGCCACCTCGCGGGGGACTACCGCGTCGTGGCGCACCGACAGCTCATT GCCTCTGAGAGCCCCGGCCGGAACCTCTACAACCAGATACGACGCTGGCC TGAGTGGCTGGAGAACGTGGACTCCATCAAGAACGCGTAA.
A gene comprising a DNA including a nucleotide sequence which codes a protein having peptidoglycan recognition activity and is capable of hybridizing with the DNA having the following nucleotide sequence (SEQ ID NO:
- 5) under stringent conditions:
- 21 ATGGCCCGCCTCCACTCGGCAGTTGTACTCGCGCTCGCTCTCAGCTCGCT TCTCACAGAAATAGCAGCCGATTGCGACGTCGTCAGTAAAAAGCAATGGG ACGGTTTGATCCCGGTGCACGTGTCGTACCTGGCGCGGCCCGTGAGCCTC GTCATCGTCCAGCACACAGTCACACCCTTCTGCAGGACGGACGCTGGCTG CGAGAGCTCGTGCGATATCCAGACCAACCACATGGAGGCCTTGCAATACT GGGACATCGGACCCTCGTTCCTGGTGGGAGGTAACGGCAAGGTGTACGAG GGCTCCGGCTGGCTGCACGTCGGCGCGCACACCTACGGGTACAACTCGAC GTCCATCGGAGTCGCATTCATCGGCAACTTCAACACGGACGAGCCGAGCG GCGCGATGCTGGAGGCGCTGCGGTCGCTGCTGCGCTGCGGCGTGGAGCGC GGCCACCTCGCGGGGGACTACCGCGTCGTGCGCACCGACAGCTCATTGCC TCTGAGAGCCCCGGCCGGAAGCTCTACAACCAGATACGACGCTGGCCTGA GTGGCTGGAGAACGTGGACTCCATCAAGAACGCGTAA.
Dependencies are inferred from the claim text.