Transmembrane protein as a downstream target of neurotrophin and ephrin receptor tyrosine kinases, DNA encoding same and monoclonal antibodies thereto
AssigneeNew York University
InventorsMoses V. Chao, Haeyoung Kong
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Abstract
The present invention relates to an ankyrin repeat-rich membrane spanning (ARMS) polypeptide which is a downstream target of neurotrophin and ephrin receptor tyrosine kinases. This ARMS polypeptide contains 1715 amino acid residues including four transmembrane domains, multiple ankyrin repeats, a sterile α motif domain, and a PDZ-binding motif. Immunofluorescence studies with anti-ARMS antibodies have found ARMS to be localized discreetly to the growth cone of neurons.
Claims34
An isolated polypeptide, which associates with TrkA and p75 neurotrophin receptors, is a target for phosphorylation by neurotrophin and ephrin receptor tyrosine kinases, enhances neurotransmitter release, and modulates the clustering of proteins involved in ion channel formation, comprising the amino acid sequence of:
- (A) SEQ ID NO:2;
- (B) SEQ ID NO:4;
- (C) a fragment of the polypeptide of SEQ ID NO:2;
- (D) a fragment of the polypeptide of SEQ ID NO:4;
- (E) a variant polypeptide which is at least 95% identical to SEQ ID NO:2;
- (F) a variant polypeptide which is at least 95% identical to SEQ ID NO:4;
- or
- (G) a functional derivative or a salt of (A), (B), (C), (D), (E), or (F)
- wherein said fragments (C) and (D) and said variants (E) and (F) have the properties of associating with TrkA and p75 neurotrophin receptors, being a target for phosphorylation by neurotrophin and ephrin receptor tyrosine kinase, enhancing neurotransmitter release, and modulating the clustering of proteins involved in ion channel formation.
An isolated nucleic acid, which specifically hybridizes under stringent conditions to the complement of either SEQ ID NO:1 or SEQ ID NO:3.
Dependencies are inferred from the claim text.