21956 and 25856, novel human aminiopeptidases and uses thereof
OwnerRosana Kapeller-Libermanninventor
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Abstract
The invention provides isolated nucleic acids molecules, designated AP nucleic acid molecules, which encode novel AP-related aminopeptidase molecules. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing AP nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which an AP gene has been introduced or disrupted. The invention still further provides isolated AP proteins, fusion proteins, antigenic peptides and anti-AP antibodies. Diagnostic methods utilizing compositions of the invention are also provided.
Claims26
An isolated nucleic acid molecule selected from the group consisting of:
- (a) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO:1 or SEQ ID NO:4;
- and
- (b) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO:3 or SEQ ID NO:6.
An isolated nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:5.
An isolated nucleic acid molecule comprising the nucleotide sequence contained in the plasmid deposited with ATCC® as Accession Number ______.
An isolated nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:5.
An isolated nucleic acid molecule selected from the group consisting of:
- a) a nucleic acid molecule comprising a nucleotide sequence which is at least 60% identical to the nucleotide sequence of SEQ ID NO:1 or 3, or SEQ ID NO:4 or 6, or a complement thereof;
- b) a nucleic acid molecule comprising a fragment of at least 97 nucleotides of a nucleic acid comprising the nucleotide sequence of SEQ ID NO:1 or 3, or a fragment of at least 45 nucleotides of a nucleic acid comprising the nucleotide sequence of SEQ ID NO:4 or 6, or a complement thereof;
- c) a nucleic acid molecule comprising nucleotide residues 803-1101 or 1547-1626 of SEQ ID NO:4;
- d) a nucleic acid molecule comprising nucleotide residues 12432-3238 or 74-342 of SEQ ID NO:1;
- e) a nucleic acid molecule which encodes a polypeptide comprising an amino acid sequence at least about 60% identical to the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:5;
- and
- f) a nucleic acid molecule which encodes a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:5, wherein the fragment comprises at least 32 contiguous amino acid residues of the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:5.
An isolated polypeptide selected from the group consisting of:
- a) a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:5, wherein the fragment comprises at least 15 contiguous amino acids of SEQ ID NO:2 or SEQ ID NO:5;
- b) a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:5, wherein the polypeptide is encoded by a nucleic acid molecule which hybridizes to a nucleic acid molecule consisting of SEQ ID NO:1 or 3, or SEQ ID NO:4 or 6, under stringent conditions;
- c) a polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least 60% identical to a nucleic acid comprising the nucleotide sequence of SEQ ID NO:1, 3, 4, or 6;
- and
- d) a polypeptide comprising an amino acid sequence which is at least 60% identical to the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:5.
Dependencies are inferred from the claim text.