US 2003/0166047 A1Published application

Lgr6 nucleic acids and uses thereof

AssigneeMillennium Pharmaceuticals, Inc.

InventorWei Gu

Life of this patent

Estimating the term…

Abstract

Novel G-protein coupled receptor molecules, designated LGR6 polypeptides, proteins, and nucleic acid molecules, are disclosed. In addition to isolated, LGR6 proteins, the invention further provides isolated LGR6 fusion proteins, antigenic peptides and anti-LGR6 antibodies. The invention also provides LGR6 nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals in which an LGR6 gene has been introduced or disrupted. Diagnostic, screening and therapeutic methods utilizing compositions of the invention are also provided.

Claims22

Dependent claims

An isolated nucleic acid molecule selected from the group consisting of:

a) a nucleic acid molecule comprising a nucleotide sequence which is at least about 60% identical to the nucleotide sequence of SEQ ID NO:
1, SEQ ID NO:
3, SEQ ID NO:
4, SEQ ID NO:
6, SEQ ID NO:
7, SEQ ID NO:
9, SEQ ID NO:
10, SEQ ID NO:
12, or the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, ______ or ______ a complement thereof;
b) a nucleic acid molecule comprising a fragment of at least 439 nucleotides of the nucleotide sequence of SEQ ID NO:
1, SEQ ID NO:
3, the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, or a complement thereof;
c) a nucleic acid molecule comprising a fragment of at least 481 nucleotides of the nucleotide sequence of SEQ ID NO:
4, SEQ ID NO:
6, the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, or a complement thereof;
d) a nucleic acid molecule comprising a fragment of at least 2175 nucleotides of the nucleotide sequence of SEQ ID NO:
7, SEQ ID NO:
9, the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, or a complement thereof;
e) a nucleic acid molecule comprising a fragment of at least 439 (CHECK NUMBER) nucleotides of the nucleotide sequence of SEQ ID NO:
10, SEQ ID NO:
12, the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, or a complement thereof;
f) a nucleic acid molecule which encodes a polypeptide comprising an amino acid sequence of at least about 60% homologous to the amino acid sequence of SEQ ID NO:
2, SEQ ID NO:
5, SEQ ID NO:
8, SEQ ID NO:
11, or an amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, ______ or ______;
g) a nucleic acid molecule which encodes a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:
2 or SEQ ID NO:
5 or SEQ ID NO:
8, or SEQ ID NO:
11, wherein the fragment comprises at least 15 contiguous amino acids of SEQ ID NO:
2, SEQ ID NO:
5, SEQ ID NO:
8, SEQ ID NO:
11, or the polypeptide encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, ______ or ______;
and
h) a nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:
2, SEQ ID NO:
5, SEQ ID NO:
8, SEQ ID NO:
11, or an amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, ______ or ______, wherein the nucleic acid molecule hybridizes to a nucleic acid molecule comprising SEQ ID NO:
1, SEQ ID NO:
3, SEQ ID NO:
4, SEQ ID NO:
6, SEQ ID NO:
7, SEQ ID NO:
9, SEQ ID NO:
10, SEQ ID NO:
12, or a complement thereof under stringent conditions.

46 more elements

Dependencies are inferred from the claim text.

Facts

Application: 09/851,595Filed 8 May 2001
Priority: 6 May 1999
Published: 4 Sep 2003
Term ends: Not estimatedThe source records don’t support an estimate.

Classification11 codes

C07K 14/723
For hormones: g protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
C07K 14/705
From animals; from humans: receptors; Cell surface antigens; Cell surface determinants
A01K 2217/05
Genetically modified animals: animals comprising random inserted nucleic acids
A61K 38/00
Medicinal preparations containing peptides

Family

Application60/132,896Us provisional application · filed 6 May 1999
Application09/566,588Continuation in part · filed 8 May 2000
Application09/851,595Continuation in part · filed 8 May 2001
US 2003/0166047 A1This patent

From the US filing history. Foreign family members aren’t listed.

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Abstract

An isolated nucleic acid molecule selected from the group consisting of:

a) a nucleic acid molecule comprising a nucleotide sequence which is at least about 60% identical to the nucleotide sequence of SEQ ID NO:
1, SEQ ID NO:
3, SEQ ID NO:
4, SEQ ID NO:
6, SEQ ID NO:
7, SEQ ID NO:
9, SEQ ID NO:
10, SEQ ID NO:
12, or the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, ______ or ______ a complement thereof;
b) a nucleic acid molecule comprising a fragment of at least 439 nucleotides of the nucleotide sequence of SEQ ID NO:
1, SEQ ID NO:
3, the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, or a complement thereof;
c) a nucleic acid molecule comprising a fragment of at least 481 nucleotides of the nucleotide sequence of SEQ ID NO:
4, SEQ ID NO:
6, the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, or a complement thereof;
d) a nucleic acid molecule comprising a fragment of at least 2175 nucleotides of the nucleotide sequence of SEQ ID NO:
7, SEQ ID NO:
9, the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, or a complement thereof;
e) a nucleic acid molecule comprising a fragment of at least 439 (CHECK NUMBER) nucleotides of the nucleotide sequence of SEQ ID NO:
10, SEQ ID NO:
12, the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, or a complement thereof;
f) a nucleic acid molecule which encodes a polypeptide comprising an amino acid sequence of at least about 60% homologous to the amino acid sequence of SEQ ID NO:
2, SEQ ID NO:
5, SEQ ID NO:
8, SEQ ID NO:
11, or an amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, ______ or ______;
g) a nucleic acid molecule which encodes a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:
2 or SEQ ID NO:
5 or SEQ ID NO:
8, or SEQ ID NO:
11, wherein the fragment comprises at least 15 contiguous amino acids of SEQ ID NO:
2, SEQ ID NO:
5, SEQ ID NO:
8, SEQ ID NO:
11, or the polypeptide encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, ______ or ______;
and
h) a nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:
2, SEQ ID NO:
5, SEQ ID NO:
8, SEQ ID NO:
11, or an amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number ______, ______ or ______, wherein the nucleic acid molecule hybridizes to a nucleic acid molecule comprising SEQ ID NO:
1, SEQ ID NO:
3, SEQ ID NO:
4, SEQ ID NO:
6, SEQ ID NO:
7, SEQ ID NO:
9, SEQ ID NO:
10, SEQ ID NO:
12, or a complement thereof under stringent conditions.

46 more elements

Facts

Application

09/851,595Filed 8 May 2001

Priority

6 May 1999

Published

4 Sep 2003

Term ends

Not estimatedThe source records don’t support an estimate.

Classification11 codes

C07K 14/723
For hormones: g protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
C07K 14/705
From animals; from humans: receptors; Cell surface antigens; Cell surface determinants
A01K 2217/05
Genetically modified animals: animals comprising random inserted nucleic acids
A61K 38/00
Medicinal preparations containing peptides