Protease and an aminopeptidase associated with development of benign prostatic hyperplasia (BPH)
OwnerStephen D. Mikolajczyk, Harry G. Rittenhouseinventors
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Abstract
Methods for identification and isolation of a benign prostate specific antigen (BPSA) protease and BPSA aminopeptidase are provided. The BPSA protease of the present invention preferentially cleaves prostate specific antigen (PSA) at residue Lys182 and does not react with the residue Arg85. The BPSA aminopeptidase cleaves BPSA between residues Ile1 and Val2 and between residues Lys146 and Leu147, but does not cleave BPSA at Ser183.
Claims18
A method for identification and isolation of a benign prostate specific antigen (BPSA) protease, the method comprising:
- (a) providing an extract from a source with elevated BPSA;
- (b) screening the extract for a BPSA protease that is capable of cleaving PSA of SEQ ID NO:1 at residue Lys182 but not at residue Arg85;
- and
- (c) isolating the BPSA protease from the extract if it is present.
A method for identification and isolation of a BPSA aminopeptidase, comprising:
- (a) providing an extract from a source with elevated BPSA;
- (b) screening the extract for a BPSA aminopeptidase that is capable of cleaving BPSA between residues Ile1 and Val2, and between residues Lys146 and Leu147;
- and
- (c) isolating the BPSA aminopeptidase from the extract if it is present.
A method for detecting the presence of a BPSA protease in a sample, the method comprising:
- (a) providing a sample suspected of containing the BPSA protease;
- (b) contacting the sample with PSA under conditions sufficient to allow cleavage of PSA by the BPSA protease to form BPSA;
- and
- (c) detecting the presence of BPSA, wherein the presence of BPSA indicates the presence of the BPSA protease.
A method of detecting the presence of a BPSA aminopeptidase in a sample, the method comprising:
- (a) providing a sample suspected of containing the BPSA aminopeptidase;
- (b) contacting the sample with BPSA under conditions sufficient to allow cleavage of BPSA by the BPSA aminopeptidase to cleave BPSA between residues Ile1 and Val2, and between residues Lys146 and Leu147 to produce peptides of SEQ ID NO:2 and SEQ ID NO:3;
- and
- (c) detecting the presence of the peptides of SEQ ID NO:2 and SEQ ID NO:3 wherein the presence of said peptides indicates the presence of the BPSA aminopeptidase.
Dependencies are inferred from the claim text.