Assay for anti-INGAP antibodies
AssigneeGMP Endotherapeutics, Inc.
InventorsAaron I. Vinik, David A. Taylor-Fishwick
Life of this patent
Estimating the term…
Abstract
A solid phase assay is used for detecting antibodies to INGAP104-118 peptide, a 15-amino acid peptide that is the biologically active portion of islet neogenesis associated protein (INGAP). The isotype of the antibodies to INGAP104-118 peptide can be determined. A kit can also be used in the detection of anti-INGAP104-118 antibodies. Endogenous autoantibodies or antibody production during therapeutic treatment of a mammal with INGAP104-118 can be monitored.
Claims25
A method for detecting antibodies to INGAP104-118 peptide in a test sample comprising:
- contacting a test sample from a mammal with INGAP104-118 peptide bound to a solid support, wherein the contacting is under conditions sufficient for binding an anti-INGAP104-118 antibody to the INGAP104-118 peptide;
- contacting the solid support with a detection antibody which specifically binds antibody molecules of all isotypes of the mammal;
- and
- determining the detection antibody bound to the solid support, wherein detection antibody bound to the solid support indicates that the test sample contains antibodies to INGAP104-118 peptide.
A method for detecting antibodies to INGAP104-118 peptide in a test sample and determining isotype of said antibodies, comprising:
- contacting a test sample from a mammal with INGAP104-118 peptide bound to a solid support, wherein the contacting is under conditions sufficient for binding an anti-INGAP104-118 antibody to the INGAP104-118 peptide;
- contacting the solid support with an isotype-specific detection antibody which specifically binds antibody molecules of one isotype of the mammal;
- and
- determining the isotype-specific detection antibody bound to the solid support, wherein isotype-specific detection antibody bound to the solid support indicates that the test sample contains antibodies to INGAP104-118 peptide and that the antibodies to INGAP104-118 peptide are of the detected isotype.
A kit for detecting an anti-INGAP104-118 antibody in a test sample of a mammal comprising:
- an INGAP104-118 peptide;
- and
- a detection antibody.
Dependencies are inferred from the claim text.