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US 2003/0166015 A1Published application
Multiplexed analysis of cell-substrate interactions
OwnerMichael A. Zarowitz, Oren E. Beske +1inventors
InventorsMichael A. Zarowitz, Oren E. Beske, Simon Goldbard
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Abstract
Systems, including methods, compositions, and kits, for multiplexed analysis of interactions between cells and substrates using coded carriers.
Claims33
A method of measuring cell-substrate interactions, comprising:
- contacting a mixture of carriers with cells, the mixture having at least two carrier classes, each carrier class including a different substrate and having a code that identifies the different substrate, the cells having an average diameter, the carriers having an average length, the average length of the carriers being greater than the average diameter of the cells;
- measuring an interaction between the cells and one or more of the carriers;
- and
- reading the code of at least one of the one or more carriers to relate the interaction to the different substrate identified by the code.
1 more element
A method of measuring cell-substrate interactions, comprising:
- contacting a mixture of carriers with cells, the mixture having at least two carrier classes, each carrier class including a different substrate and having a code that identifies the different substrate, the cells being present in numerical excess over the carriers;
- measuring an interaction between the cells and one or more of the carriers;
- and
- reading the code of at least one of the one or more carriers to relate the interaction to the different substrate identified by the code.
1 more element
A method of screening for modulators of cell-substrate interactions, comprising:
- contacting a mixture of carriers with cells, the mixture having at least two carrier classes, each carrier class including a different substrate and having a code that identifies the different substrate, the cells having an average diameter, the carriers having an average length, the average length of the carriers being greater than the average diameter of the cells;
- exposing at least one of the mixture and the cells to a candidate modulator;
- measuring an interaction between the cells and one or more of the carriers;
- and
- reading the code of at least one of the one or more carriers to relate the interaction to the different substrate identified by the code and thus to any effect of the candidate modulator on the interaction of the cells with such different substrate.
2 more elements
A kit for multiplexed analysis of cell-substrate interactions, comprising:
- a set of carriers, the set including at least two carriers classes, each carrier class having a different code and including a different extracellular matrix material, the different extracellular matrix material being identified by the different code.
A system for measuring cell-substrate interactions, comprising:
- means for contacting a mixture of carriers with cells, the mixture having at least two carrier classes, each carrier class including a different substrate and having a code that identifies the different substrate, the cells having an average diameter, the carriers having an average length, the average length of the carriers being greater than the average diameter of the cells;
- means for measuring an interaction between the cells and one or more of the carriers;
- and
- means for reading the code of at least one of the one or more carriers to relate the interaction to the different substrate identified by the code.
1 more element
Dependencies are inferred from the claim text.