Mosquito olfactory genes, polypeptides, and methods of use thereof
AssigneeVanderbilt University
InventorLaurence J. Zwiebel
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Abstract
The invention discloses polynucleotides and polypeptides of arrestin and odorant receptors. Also disclosed are methods for producing such polypeptides and methods of making antibodies. This invention also discloses a method of identifying compounds that bind to arrestins or odorant receptors. A method of identifying compounds that inhibit the binding of mosquito arrestin to a mosquito odorant receptor is also disclosed.
Claims85
A method of identifying an agent that binds to mosquito olfaction molecules, comprising:
- a) providing an isolated mosquito olfaction molecule;
- b) contacting a test agent with the isolated mosquito olfaction molecule;
- and
- c) detecting specific binding of the test agent to the isolated mosquito olfaction molecule,
- wherein the presence of specific binding identifies the test agent as a mosquito olfaction molecule binding compound.
A method of identifying a compound that inhibits binding of a mosquito arrestin to a mosquito odorant receptor, comprising:
- providing an antibody that binds to an isolated mosquito olfaction molecule;
- providing a mosquito olfaction molecule binding compound;
- providing a test sample;
- combining the mosquito olfaction molecule binding compound, the antibody, and the test sample in reaction conditions that allow a complex to form in the absence of the mosquito olfaction molecule binding compound, wherein the complex includes the mosquito arrestin and the mosquito odorant receptor;
- and
- determining whether the mosquito olfaction molecule binding compound decreases the formation of the complex, wherein a decrease indicates that the mosquito olfaction molecule binding compound is a compound that inhibits the binding of mosquito arrestin to mosquito odorant receptor.
The method of 8, wherein a GAL4 binding domain is linked to an arrestin fragment.
An isolated polynucleotide comprising a sequence selected from the group consisting of:
- a nucleotide sequence encoding a polypeptide comprising an amino acid sequence of SEQ ID NO:
- 2;
- a nucleotide sequence encoding a polypeptide comprising at least 20 consecutive residues of the amino acid sequence of SEQ ID NO:
- 2;
- a nucleotide sequence encoding a polypeptide comprising a conservatively modified amino acid sequence of SEQ ID NO:
- 2;
- and
- a nucleotide sequence that hybridizes under stringent conditions to a hybridization probe the nucleotide sequence of which consists of SEQ ID NO:
- 1, or the complement of SEQ ID NO:
- 1.
A purified polypeptide comprising a sequence selected from the group consisting of:
- an amino acid sequence of SEQ ID NO:
- 2;
- an amino acid sequence of conservatively modified SEQ ID NO:
- 2;
- and
- an amino acid sequence of SEQ ID NO:
- 2, having at least 20 consecutive residues.
An isolated polynucleotide comprising a sequence selected from the group consisting of:
- a nucleotide sequence encoding a polypeptide comprising an amino acid sequence of SEQ ID NO:
- 4;
- a nucleotide sequence encoding a polypeptide comprising at least 20 consecutive residues of the amino acid sequence of SEQ ID NO:
- 4;
- a nucleotide sequence encoding a polypeptide comprising a conservatively modified amino acid sequence of SEQ ID NO:
- 4;
- and
- a nucleotide sequence that hybridizes under stringent conditions to a hybridization probe the nucleotide sequence of which consists of SEQ ID NO:
- 3, or the complement of SEQ ID NO:
- 3.
A purified polypeptide comprising a sequence selected from the group consisting of:
- an amino acid sequence of SEQ ID NO:
- 4;
- an amino acid sequence of conservatively modified SEQ ID NO:
- 4;
- and
- an amino acid sequence of SEQ ID NO:
- 4, having at least 20 consecutive residues.
An isolated polynucleotide comprising a sequence selected from the group consisting of:
- a nucleotide sequence encoding a polypeptide comprising an amino acid sequence of SEQ ID NO:
- 6;
- a nucleotide sequence encoding a polypeptide comprising at least 20 consecutive residues of the amino acid sequence of SEQ ID NO:
- 6;
- a nucleotide sequence encoding a polypeptide comprising a conservatively modified amino acid sequence of SEQ ID NO:
- 6;
- and
- a nucleotide sequence that hybridizes under stringent conditions to a hybridization probe the nucleotide sequence of which consists of SEQ ID NO:
- 5, or the complement of SEQ ID NO:
- 5.
A purified polypeptide comprising a sequence selected from the group consisting of:
- an amino acid sequence of SEQ ID NO:
- 6;
- an amino acid sequence of conservatively modified SEQ ID NO:
- 6;
- and
- an amino acid sequence of SEQ ID NO:
- 6, having at least 20 consecutive residues.
An isolated polynucleotide comprising a sequence selected from the group consisting of:
- a nucleotide sequence encoding a polypeptide comprising an amino acid sequence of SEQ ID NO:
- 8;
- a nucleotide sequence encoding a polypeptide comprising at least 20 consecutive residues of the amino acid sequence of SEQ ID NO:
- 8;
- a nucleotide sequence encoding a polypeptide comprising a conservatively modified amino acid sequence of SEQ ID NO:
- 8;
- and
- a nucleotide sequence that hybridizes under stringent conditions to a hybridization probe the nucleotide sequence of which consists of SEQ ID NO:
- 7, or the complement of SEQ ID NO:
- 7.
A purified polypeptide comprising a sequence selected from the group consisting of:
- an amino acid sequence of SEQ ID NO:
- 8;
- an amino acid sequence of conservatively modified SEQ ID NO:
- 8;
- and
- an amino acid sequence of SEQ ID NO:
- 8, having at least 20 consecutive residues.
An isolated polynucleotide comprising a sequence selected from the group consisting of:
- a nucleotide sequence encoding a polypeptide comprising an amino acid sequence of SEQ ID NO:
- 14;
- a nucleotide sequence encoding a polypeptide comprising at least 20 consecutive residues of the amino acid sequence of SEQ ID NO:
- 14;
- a nucleotide sequence encoding a polypeptide comprising a conservatively modified amino acid sequence of SEQ ID NO:
- 14;
- and
- a nucleotide sequence that hybridizes under stringent conditions to a hybridization probe the nucleotide sequence of which consists of SEQ ID NO:
- 13, or the complement of SEQ ID NO:
- 13.
A purified polypeptide comprising a sequence selected from the group consisting of:
- an amino acid sequence of SEQ ID NO:
- 14;
- an amino acid sequence of conservatively modified SEQ ID NO:
- 14;
- and
- an amino acid sequence of SEQ ID NO:
- 14, having at least 20 consecutive residues.
An isolated polynucleotide comprising a sequence selected from the group consisting of:
- a nucleotide sequence encoding a polypeptide comprising an amino acid sequence of SEQ ID NO:
- 16;
- a nucleotide sequence encoding a polypeptide comprising at least 20 consecutive residues of the amino acid sequence of SEQ ID NO:
- 16;
- a nucleotide sequence encoding a polypeptide comprising a conservatively modified amino acid sequence of SEQ ID NO:
- 16;
- and
- a nucleotide sequence that hybridizes under stringent conditions to a hybridization probe the nucleotide sequence of which consists of SEQ ID NO:
- 15, or the complement of SEQ ID NO:
- 15.
A purified polypeptide comprising a sequence selected from the group consisting of:
- an amino acid sequence of SEQ ID NO:
- 16;
- an amino acid sequence of conservatively modified SEQ ID NO:
- 16;
- and
- an amino acid sequence of SEQ ID NO:
- 16, having at least 20 consecutive residues.
An isolated polynucleotide comprising a sequence selected from the group consisting of:
- a nucleotide sequence encoding a polypeptide comprising an amino acid sequence of SEQ ID NO:
- 18;
- a nucleotide sequence encoding a polypeptide comprising at least 20 consecutive residues of the amino acid sequence of SEQ ID NO:
- 18;
- a nucleotide sequence encoding a polypeptide comprising a conservatively modified amino acid sequence of SEQ ID NO:
- 18;
- and
- a nucleotide sequence that hybridizes under stringent conditions to a hybridization probe the nucleotide sequence of which consists of SEQ ID NO:
- 17, or the complement of SEQ ID NO:
- 17.
A purified polypeptide comprising a sequence selected from the group consisting of:
- an amino acid sequence of SEQ ID NO:
- 18;
- an amino acid sequence of conservatively modified SEQ ID NO:
- 18;
- and
- an amino acid sequence of SEQ ID NO:
- 18, having at least 20 consecutive residues.
An isolated polynucleotide comprising a sequence selected from the group consisting of:
- a nucleotide sequence encoding a polypeptide comprising an amino acid sequence of SEQ ID NO:
- 20;
- a nucleotide sequence encoding a polypeptide comprising at least 20 consecutive residues of the amino acid sequence of SEQ ID NO:
- 20;
- a nucleotide sequence encoding a polypeptide comprising a conservatively modified amino acid sequence of SEQ ID NO:
- 20;
- and
- a nucleotide sequence that hybridizes under stringent conditions to a hybridization probe the nucleotide sequence of which consists of SEQ ID NO:
- 19, or the complement of SEQ ID NO:
- 19.
A purified polypeptide comprising a sequence selected from the group consisting of:
- an amino acid sequence of SEQ ID NO:
- 20;
- an amino acid sequence of conservatively modified SEQ ID NO:
- 20;
- and
- an amino acid sequence of SEQ ID NO:
- 20, having at least 20 consecutive residues.
A method of modulating arrestin 1 biological activity, the method comprising:
- administering an arrestin 1 biological activity-modulating amount of a mosquito olfaction molecule binding compound;
- contacting the arrestin 1 with the mosquito olfaction molecule binding compound;
- and
- modulating arrestin 1 biological activity through the arrestin 1 contact with the mosquito olfaction molecule binding compound.
Dependencies are inferred from the claim text.