DNA molecules encoding the melanocortin 4 receptor protein from rhesus monkey
AssigneeMerck & Co., Inc.
InventorsDouglas J. MacNeil, David H. Weinberg, Leonardus H. T. Van Der Ploeg
Life of this patent
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Abstract
The present invention relates to rhesus monkey DNA molecules encoding the melanocortin-4 receptor protein, recombinant vectors comprising DNA molecules encoding rhesus MC-4R, recombinant host cells which contain a recombinant vectors encoding rhesus MC-4R, the rhesus MC-4R protein encoded by the DNA molecule, and methods of identifying selective agonists and antagonists of rhesus MC-4R.
Claims39
A purified nucleic acid molecule encoding a rhesus melanocortin 4 receptor protein which comprises the nucleotide sequence
- 5 GAATTCTCCT GCCAGCATGG TGAACTCCAC CCACCGTGGG ATGCACGCTT CTCTGCACCT CTGGAACCGC AGCAGCCACA GACTGCACAG CAATGCCAGT GAGTCCCTTG GAAAAGGCTA CTCTGATGGA GGGTGCTACG AGCAACTTTT TGTCTCTCCT GAGGTGTTTG TGACACTGGG TGTCATCAGC TTGTTGGAGA ATATCTTAGT GATTGTGGCA ATAGCCAAGA ACAAGAATCT GCATTCACCC ATGTACTTTT TCATCTGCAG CCTGGCTGTG GCTGATATGC TGGTGAGCGT TTCAAATGGA TCAGAAACCA TTGTCATCAC CCTATTAAAC AGTACAGATA CGGACACACA GAGTTTCACA GTGAACATTG ATAATGTTAT TGACTCAGTG ATCTGTAGCT CCTTGCTTGC ATCCATTTGC AGCCTGCTTT CAATTGCAGT GGACAGGTAC TTTACTATCT TCTATGCTCT TCAGTACCAT AACATTATGA CAGTTAAGCG GGTTGGGATC ATCATAAGTT GTATCTGGGC AGCTTGCACG GTTTCAGGCA TTTTGTTCAT CATTTACTCA GATAGTAGTG CTGTCATCAT CTGCCTCATC ACCATGTTCT TCACCATGTT GGCTCTCATG GCTTCTCTCT ATGTCCACAT GTTCCTGATG GCCAGGCTTC ACATTAAGAG GATTGCTGTC CTCCCCGGCA CTGGTGCCAT CCGCCAAGGC GCCMiTATGA AGGGAGCGAT TACTTTGACC ATCCTGATTG GCGTCTTTGT TGTCTGCTGG CCCCCATTCT TCCTCCACTT AATATTCTAC ATCTCTTGTC CTCAGAATCC ATATTGTGTG TGCTTCATGT CTCACTTTAA CTTGTATCTC ATACTGATCA TGTGTAATTC AGTCATCGAT CCTCTGATTT ATGCACTCCG GAGTCAAGAA CTAAGGAAAA CCTTCAAAGA GATCATCTGT TGCTATCCCC TGGGAGGCCT ATGTGACTTG TCTAGCAGAT ATTAAATGGG GACAGAGCAC, as set forth in SEQ ID NO:1.
A purified DNA molecule encoding rhesus melanocortin 4 receptor protein wherein said DNA molecule encodes a protein comprising the amino acid sequence
- 6 MVNSTHRGMH ASLHLWNRSS HRLHSNASES LGKGYSDGCC YEQLFVSPEV FVTLGVISLL ENILVIVAIA KNKNLHSPMY FFICSLAVAD MLVSVSNGSE TIVITLLNST DTDTQSFTVN IDNVIDSVIC SSLLASICSL LSIAVDRYFT IFYALQYHNI MTVKRVGIII SCIWAACTVS GILFIIYSDS SAVIICLITM FFTMLALMAS LYVHMFLMAR LHIKRIAVLP GTGAIRQGAN MKGAITLTIL IGVFVVCWAP FFLHLIFYIS CPQNPYCVCF MSHFNLYLIL IMCNSVIDPL IYALRSQELR KTFKEIICCY PLGGLCDLSS RY, as set forth in a three-letter abbreviation in SEQ ID NO:2.
A purified DNA molecule encoding a rhesus melanocortin 4 receptor protein which consists of the nucleotide sequence
- 7 GAATTCTCCT GCCAGCATCG TGAACTCCAC CCACCGTGGG ATGCACGCTT CTCTGCACCT CTGGAACCGC AGCAGCCACA GACTGCACAG CAATGCCAGT GAGTCCCTTG GAAAAGGCTA CTCTGATGGA GCGTGCTACG AGCAACTTTT TGTCTCTCCT GAGGTGTTTG TGACACTGGG TGTCATCAGC TTGTTGGAGA ATATCTTAGT GATTGTGGCA ATAGCCAAGA ACAAGAATCT GCATTCACCC ATGTACTTTT TCATCTGCAG CCTGGCTGTG GCTGATATGC TGGTGAGCGT TTCAAATGGA TCAGAAACCA TTGTCATCAC CCTATTAAAC AGTACAGATA CGGACACACA GAGTTTCACA GTGAACATTG ATAATGTTAT TGACTCAGTG ATCTGTAGCT CCTTGCTTGC ATCCATTTGC AGCCTGCTTT CAATTGCAGT GGACAGGTAC TTTACTATCT TCTATGCTCT TCAGTACCAT AACATTATGA CAGTTAAGCG GGTTGGGATC ATCATAAGTT GTATCTGGGC AGCTTGCACG GTTTCAGGCA TTTTGTTCAT CATTTACTCA GATAGTAGTG CTGTCATCAT CTGCCTCATC ACCATGTTCT TCACCATGTT GGCTCTCATG GCTTCTCTCT ATGTCCACAT GTTCCTGATG GCCAGGCTTC ACATTAAGAG GATTGCTGTC CTCCCCGGCA CTGGTGCCAT CCGCCAAGGC GCCAATATGA AGGGAGCGAT TACTTTGACC ATCCTGATTG GCGTCTTTGT TGTCTGCTGG GCCCCATTCT TCCTCCACTT AATATTCTAC ATCTCTTGTC CTCAGAATCC ATATTGTGTG TGCTTCATGT CTCACTTTAA CTTGTATCTC ATACTGATCA TGTGTAATTC AGTCATCGAT CCTCTGATTT ATGCACTCCG GAGTCAAGAA CTAAGGAAAA CCTTCAAAGA GATCATCTGT TGCTATCCCC TGGGAGGCCT ATGTGACTTG TCTAGCAGAT ATTAAATGGC GACAGAGCAC, as set forth in SEQ ID NO:1.
A purified DNA molecule which encodes a MC-4R protein which consists of the amino acid sequence
- 8 MVNSTHRGMH ASLHLWNRSS HRLHSNASES LGKGYSDGGC YEQLFVSPEV FVTLGVISLL ENILVIVAIA KNKNLHSPMY FFTCSLAVAD MLVSVSNGSE TIVITLLNST DTDTQSFTVN IDNVIDSVIC SSLLASICSL LSIAVDRYFT IFYALQYHNI MTVKRVGIII SCIWAACTVS GILFTIYSDS SAVIICLITM FFTMLALMAS LYVHMFLMAR LHIKRIAVLP GTGAIRQGAN MKGAITLTIL IGVFVVCWAP FFLHLIFYIS CPQNPYCVCF MSHFNLYLIL IMCNSVIDPL IYALRSQELR KTFKETTCCY PLGGLCDLSS RY, as set forth in three letter abbreviation in SEQ ID NO:2.
A purified rhesus melanocortin 4 receptor protein which comprises the amino acid sequence as follows:
- 9 MVNSTHRGMH ASLHLWNRSS HRLHSNASES LGKGYSDGGC YEQLFVSPEV FVTLGVTSLL ENILVIVAIA KNKNLHSPMY FFICSLAVAD MLVSVSNGSE TIVITLLNST DTDTQSFTVN IDNVIDSVIC SSLLASTCSL LSIAVDRYFT IFYALQYHNI MTVKRVGIII SCIWAACTVS GILFIIYSDS SAVIICLITM FFTMLALMAS LYVHMFLMAR LHIKRIAVLP GTGAIRQGAN MKGAITLTIL IGVFVVCWAP FFLHLIFYIS CPQNPYCVCF MSHFNLYLIL IMCNSVIDPL IYALRSQELR KTFKEIICCY PLGGLCDLSS RY, as set forth in a three-letter abbreviation in SEQ ID NO:2.
A purified rhesus melanocortin 4 receptor protein which consists of the amino acid sequence as set forth in SEQ ID NO:2.
A method for determining whether a substance is capable of binding to rhMC-4R comprising:
- (a) providing test cells by transfecting cells with an expression vector that directs the expression of rhMC-4R in the cells;
- (b) exposing the test cells to the substance;
- (c) measuring the amount of binding of the substance to rhMC-4R;
- and,
- (d) comparing the amount of binding of the substance to rhMC-4R in the test cells with the amount of binding of the substance to control cells that have not been transfected with rhMC-4R.
A method for determining whether a substance is capable of activating rhMC-4R comprising:
- (a) providing test cells by transfecting cells with an expression vector that directs the expression of rhMC-4R in the cells;
- (b) exposing the test cells to the substance;
- (c) measuring the amount of accumulated intracellular cAMP;
- and,
- (d) comparing the amount of cAMP in the test cells in response to the substance with the amount of cAMP in test cells that have not been exposed to the substance.
A method of identifying a substance which modulates MC-4R receptor activity, comprising:
- (a) combining a test substance in the presence and absence of a MC-4R receptor protein wherein said MC-4R receptor protein comprises the amino acid sequence as set forth in SEQ ID NO:2;
- and,
- (b) measuring and comparing the effect of the test substance in the presence and absence of the MC-4R receptor protein.
An antibody that binds specifically to MC-4R protein wherein the MC-4R receptor protein comprises the amino acid sequence as set forth in SEQ ID NO:2.
Dependencies are inferred from the claim text.