Test system for determining the activity of cyclo-nucleotide-dependent protein kinases and vasp phosphatases

An HTS-suitable test system for detecting the activity of the VASP phosphatase, comprising

• e) at least one test compound,
• f) at least one suitable VASP phosphatase substrate,
• g) at least one composition which is to be incubated and which contains VASP phosphatase, and
• h) a suitable detection system for quantitatively determining the quantity of the dephosphorylated VASP phosphatase substrate.

A method for diagnosing by directly, and in a gel electrophoresis-independent manner, determining the cNPK activity in samples, comprising the steps of:

• m) incubating a sample in the presence of at least one composition containing a suitable cNPKS and ATP,
• n) adding at least one detection system which is suitable for quantifying the phosphorylation of the cNPKS to the composition;
• and
• o) determining the activity of the cNPK, in particular in blood extracts, cell extracts or tissue extracts or in samples which are in the form of permeabilized and/or intact cells.

A method for diagnosing by directly, and in a gel electrophoresis-independent manner, determining the VASP phosphatase activity in samples, comprising the steps of:

• p) incubating a sample in the presence of at least one composition containing a suitable VASP phosphatase substrate,
• q) adding at least one detection system which is suitable for quantifying the dephosphorylation of the VASP phosphatase substrate to the composition;
• and
• r) determining the activity of the VASP phosphatase, in particular in blood extracts, cell extracts or tissue extracts or in samples which are in the form of permeabilized and/or intact cells.