Back to results
US 2003/0166001 A1Published application
Toll-like receptor 3 signaling agonists and antagonists
OwnerGrayson B. Lipfordinventor
Life of this patent
Estimating the term…
Abstract
Compositions and methods are provided to identify, characterize, and optimize immunostimulatory compounds, their agonists and antagonists, working through TLR3.
Claims34
A screening method for identifying an immunostimulatory compound, comprising:
- contacting a functional TLR3 with a test compound under conditions which, in absence of the test compound, permit a negative control response mediated by a TLR3 signal transduction pathway;
- detecting a test response mediated by the TLR3 signal transduction pathway;
- and
- determining the test compound is an immunostimulatory compound when the test response exceeds the negative control response.
1 more element
A screening method for identifying an immunostimulatory compound, comprising:
- contacting a functional TLR3 with a test compound under conditions which, in presence of a reference immunostimulatory compound, permit a reference response mediated by a TLR3 signal transduction pathway;
- detecting a test response mediated by the TLR3 signal transduction pathway;
- and
- determining the test compound is an immunostimulatory compound when the test response equals or exceeds the reference response.
1 more element
A screening method for identifying a compound that modulates TLR3 signaling activity, comprising:
- contacting a functional TLR3 with a test compound and a reference immunostimulatory compound under conditions which, in presence of the reference immunostimulatory compound alone, permit a reference response mediated by a TLR3 signal transduction pathway;
- detecting a test-reference response mediated by the TLR3 signal transduction pathway;
- determining the test compound is an agonist of TLR3 signaling activity when the test-reference response exceeds the reference response;
- and
- determining the test compound is an antagonist of TLR3 signaling activity when the reference response exceeds the test-reference response.
2 more elements
A screening method for identifying species specificity of an immunostimulatory compound, comprising:
- measuring a first species-specific response mediated by a TLR3 signal transduction pathway when a functional TLR3 of a first species is contacted with a test compound;
- measuring a second species-specific response mediated by the TLR3 signal transduction pathway when a functional TLR3 of a second species is contacted with the test compound;
- and
- comparing the first species-specific response with the second species-specific response.
1 more element
Dependencies are inferred from the claim text.