Human antibodies as detection reagents
AssigneesBiosite, Inc., Medarex, Inc.
InventorsJoe Buechler, Gunars Valkirs, Jeff Gray, Nils Lonberg
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Abstract
The invention provides in vitro methods of detection using human antibodies. The methods are particularly useful for analyzing human samples containing HAMA or heterophilic antibodies. A human antibody can bind to an analyte in such samples without binding to HAMA or heterophilic antibodies present in the samples. The methods can be effected using a sandwich format among others.
Claims54
A method of detecting an analyte, comprising:
- contacting a sample with a human antibody immobilized to a support, the antibody having an affinity of at least 108M−1 for an analyte,
- detecting binding of the human antibody to the analyte indicating presence and/or amount of the analyte in the sample.
A plurality of human antibodies each having an affinity of at least is 108 M−1 for a different corresponding analyte, the different human antibodies occupying different known locations on one or more supports.
A method of detecting an analyte in a human sample containing human antibodies that specifically bind to antibodies from a nonhuman species, comprising:
- contacting the sample with a human antibody, wherein the human antibody specifically binds to the analyte without specifically binding to the human antibodies that specifically bind to antibodies from a nonhuman species, and
- detecting the binding between the human antibody and the analyte to indicate presence of the analyte.
A method of detecting an analyte in a sample, comprising
- contacting the sample with a first human antibody immobilized to a solid phase, and a second human antibody in solution, wherein the first and second antibodies bind to different epitopes of the analyte if present in the sample
- detecting binding of the analyte to the first and/or second antibodies, binding indicating presence of the analyte in the sample.
Dependencies are inferred from the claim text.