Peptide mimotope to mycotoxin deoxynivalenol and uses thereof
AssigneeBoard of Trustees operating Michigan State University
InventorsLynn Patrick Hart, James J. Pestka, Qiaoping Yuan
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Abstract
The present invention provides a peptide mimotope of the non-peptide mycotoxin deoxynivalenol. In particular, the peptide mimotope competes with deoxynivalenol for binding to a monoclonal antibody and is antagonistic to the inhibitory effects of deoxynivalenol on in vitro protein synthesis. The present invention also provides a method that uses the peptide mimotope to determine whether corn, grains or mixed feed is contaminated with fungi that produces deoxynivalenol. The present invention further provides transgenic plants resistant to deoxynivalenol.
Claims56
A peptide mimotope of deoxynivalenol (DON) comprising amino acid sequence SWGPX1PX2 which binds to a monoclonal antibody against the DON wherein X1 is L, F, or analog thereof and X2 is each any amino acid or analog thereof.
A peptide mimotope of deoxynivalenol (DON) comprising the amino acid sequence SWGPFPF which binds to a monoclonal antibody against the DON.
A peptide mimotope of deoxynivalenol (DON) comprising the amino acid sequence SWGPLPF which binds to a monoclonal antibody against the DON.
A peptide mimotope of deoxynivalenol (DON) comprising the amino acid sequence SWGPFPFGGGSC which binds to a monoclonal antibody against the DON.
A nucleic acid encoding a nucleic acid that encodes a peptide mimotope of DON, which binds to a monoclonal antibody against DON and is antagonistic to the inhibitory effects of DON on in vitro protein synthesis, comprising an amino acid sequence selected from the group consisting of SWGPLPF, SWGPFPF, and SWGPFPFGGGSC.
A clone in a microorganism expressing a peptide mimotope of deoxynivalenol (DON) comprising amino acid sequence SWGPX1PX2 which binds to a monoclonal antibody against the DON wherein X1 is L, F, or analog thereof and X2 is each any amino acid or analog thereof.
A transgenic plant containing a nucleic acid that encodes a peptide mimotope of deoxynivalenol (DON) wherein the peptide mimotope of DON binds to a monoclonal antibody against DON and is antagonistic to the inhibitory effects of DON on in vitro protein synthesis.
A peptide mimotope of deoxynivalenol (DON) which competes with DON for binding to a monoclonal antibody, is antagonistic to the inhibitory effects of the DON on in vitro protein synthesis, and does not elicit antibodies in mice against the DON.
In a method for determining whether a sample contains deoxynivalenol (DON) which comprises providing monoclonal antibody against the DON, reacting the monoclonal antibody with the sample in a reaction mixture containing a labeled DON as a competitor, and determining whether the sample contains the DON, the improvement comprises providing as the competitor a peptide mimotope of the DON which is bound by the monoclonal antibody.
A method for determining whether a sample contains deoxynivalenol (DON) which comprises:
- (a) incubating in a reaction the sample, a monoclonal antibody against the DON, and a peptide mimotope of the DON which is a competitor of the DON for the monoclonal antibody;
- (b) detecting in the reaction a complex consisting of the DON bound by the monoclonal antibody and a complex formed by the peptide mimotope and monoclonal antibody;
- and
- (c) comparing an amount of each of the complexes wherein a decrease in the amount of the complex comprising the peptide mimotope indicates the sample contains the DON.
A kit for determining whether a sample contains deoxynivalenol (DON) comprising:
- (a) a monoclonal antibody against the DON;
- (b) a peptide mimotope of the DON which is a competitor of the DON for binding to the monoclonal antibody;
- and
- (c) instructions for using the kit.
A method for making a plant resistant to dexoynivalenol (DON) comprising introducing into the plant's genome a nucleic acid that encodes a peptide mimotope of the DON which is operably linked to a transcription promoter, wherein the peptide mimotope is antagonistic to the inhibitory effects of DON on in vitro protein synthesis.
A transgenic plant comprising a nucleic acid that encodes a peptide mimotope of the DON which is operably linked to a transcription promoter, wherein the peptide mimotope is antagonistic to the inhibitory effects of DON on in vitro protein synthesis.
A method for treating an organism exposed to deoxynivalenol (DON) comprising treating the organism with a peptide mimotope of the DON which is antagonistic to the inhibitory effects of the DON on in vitro protein synthesis.
Dependencies are inferred from the claim text.