Compositions and methods for surface imprinting
AssigneeAspira Biosystems, Inc.
InventorChin-Shiou Huang
Life of this patent
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Abstract
The present invention provides surface imprint compositions useful for capturing, isolating, detecting, analyzing and/or quantifying molecules in a sample. The surface imprint compositions comprise a matrix material having imprint cavities of a template molecule or molecules imprinted thereon wherein a- substantial number of the imprint cavities are located at or near the surface of the matrix material.
Claims57
A surface imprint composition comprising a matrix material defining imprint cavities of a template molecule wherein a substantial fraction of the imprint cavities are localized at or near the surface of the matrix material.
A surface imprint composition comprising a matrix material defining imprint cavities of a template molecule wherein a substantial fraction of the imprint cavities are oriented.
A method of preparing a surface imprint comprising the steps of:
- (a) forming a hardened matrix in the presence of an immobilized template molecule;
- and
- (b) removing the template molecule from the hardened matrix, yielding a surface imprint.
A method of making a surface imprint comprising the steps of:
- (a) dispersing a polymerizable compound and a conjugate molecule in a solvent system which comprises a first solvent and a second solvent which is immiscible with the first solvent such that they form a two-phase system wherein the polymerizable compound and the template moiety of the conjugate molecule partition into the same phase of the two-phase system;
- (b) polymerizing the polymerizable compound;
- and
- (c) removing the conjugate molecule.
A method of making an surface imprint array capable of capturing a plurality of different molecules, comprising the steps of:
- (a) forming a hardened matrix in the presence of an array of immobilized template molecules;
- and
- (b) removing at least two of the template molecules from the hardened matrix yielding a surface imprint array.
A method of screening a plurality of macromolecules, comprising contacting the plurality of macromolecules with a matrix, said matrix comprising an surface imprint of a template molecule wherein the template molecule is selected from a peptide consisting of 3 to 30 amino acids, a polynucleotide consisting of 3 to 30 nucleotides, and an oligosaccharide consisting of 3 to 30 saccharides, under conditions in which at least one molecule of the plurality binds the matrix.
A method of screening a plurality of macromolecules, comprising contacting the plurality of macromolecules with a plurality of matrices, said matrices comprising a plurality of surface imprints of template molecules, wherein at least two of the template molecules are unique, wherein the template molecules are selected from a peptide consisting of 3 to 30 amino acids, a polynucleotide consisting of 3 to 30 nucleotides, and an oligosaccharide consisting of 3 to 30 saccharides, and under conditions in which at least one molecule of the plurality binds a matrix.
Dependencies are inferred from the claim text.