Method for the determination of a nucleic acid using a control
AssigneeRoche Molecular Systems, Inc.
InventorStephan Jaeger
Life of this patent
Estimating the term…
Abstract
The present invention is directed to a method for the determination of a target nucleic acid using a special control nucleic acid, a method for the amplification of a partial sequence of said target nucleic acid using primers, a special control and a kit containing said control. The sequence of these control nucleic acids are at least in part parallel-complementary to the sequence of the target nucleic. These controls have similar properties as the target nucleic acid in hybridization and amplification methods, but can be well differentiated from the target nucleic acid by their different sequence.
Claims14
A method for the amplification of a target nucleic acid region in a sample comprising the step of
- amplifying a known amount of a control nucleic acid and said target nucleic acid, wherein said control nucleic acid comprises at least one contiguous sequence of at least 8 nucleotides in length essentially parallel complementary to said target nucleic acid region or to the complementary strand of said target nucleic acid region.
A method for quantitation of a target nucleic acid region comprising the steps of:
- a) amplifying said target nucleic acid region and a known amount of a control nucleic acid, wherein said control nucleic acid comprises at least one contiguous sequence of at least 8 nucleotides in length essentially parallel complementary to said target nucleic acid region or to the complementary strand of said target nucleic acid region,
- b) detecting a signal indicative for the amount of amplification product obtained from said control nucleic acid and detecting a signal indicative for the amount of amplification product obtained from said target nucleic acid,
- c) calculating the amount of said target nucleic acid using the known amount of said control nucleic acid, the signal indicative for the amount of amplification product obtained from said control nucleic acid detected in step b) and the signal indicative for the amount of amplification product obtained from said target nucleic acid detected in step b).
A control nucleic acid for use in a reaction for the amplification of a target nucleic acid region, wherein said control nucleic acid comprises at least one contiguous sequence of at least 8 nucleotides in length essentially parallel complementary to said target nucleic acid region or to the complementary strand of said target nucleic acid region.
A composition comprising a target nucleic acid and a control nucleic acid, wherein said control nucleic acid comprises at least one contiguous sequence of at least 8 nucleotides in length essentially parallel complementary to said target nucleic acid region or to the complementary strand of said target nucleic acid region.
A kit for the amplification of a target nucleic acid comprising an instruction manual, a target nucleic acid and a control nucleic acid wherein said control nucleic acid comprises at least one contiguous sequence of at least 8 nucleotides in length essentially parallel complementary to said target nucleic acid region or to the complementary strand of said target nucleic acid region.
Dependencies are inferred from the claim text.