Regulation of human pgc-1-like protein
OwnerTimothy J Smithinventor
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Abstract
Reagents which regulate human PGC-1-like protein and reagents which bind to human PGC-1-like gene products can play a role in preventing, ameliorating, or correcting dysfunctions or diseases including, but not limited to, cancer, obesity, cardiovascular disorders, diabetes, and COPD.
Claims71
An isolated polynucleotide encoding a PGC-1-like protein polypeptide and being selected from the group consisting of:
- a) a polynucleotide encoding a PGC-1-like protein polypeptide comprising an amino acid sequence selected form the group consisting of:
- amino acid sequences which are at least about 32% identical to the amino acid sequence shown in SEQ ID NO:
- 2;
- and
- the amino acid sequence shown in SEQ ID NO:
- 2.
- b) a polynucleotide comprising the sequence of SEQ ID NO:
- 1;
- c) a polynucleotide which hybridizes under stringent conditions to a polynucleotide specified in (a) and (b);
- d) a polynucleotide the sequence of which deviates from the polynucleotide sequences specified in (a) to (c) due to the degeneration of the genetic code;
- and
- e) a polynucleotide which represents a fragment, derivative or allelic variation of a polynucleotide sequence specified in (a to (d).
A cDNA encoding a polypeptide comprising the amino acid sequence shown in SEQ ID NO:2.
An expression vector comprising a polynucleotide which encodes a polypeptide comprising the amino acid sequence shown in SEQ ID NO:2.
A host cell comprising an expression vector which encodes a polypeptide comprising the amino acid sequence shown in SEQ ID NO:2.
A purified polypeptide comprising the amino acid sequence shown in SEQ ID NO:2.
A fusion protein comprising a polypeptide having the amino acid sequence shown in SEQ ID NO:2.
A method of producing a polypeptide comprising the amino acid sequence shown in SEQ ID NO:2, comprising the steps of:
- culturing a host cell comprising an expression vector which encodes the polypeptide under conditions whereby the polypeptide is expressed;
- and
- isolating the polypeptide.
A method of detecting a coding sequence for a polypeptide comprising the amino acid sequence shown in SEQ ID NO:2, comprising the steps of:
- hybridizing a polynucleotide comprising 11 contiguous nucleotides of SEQ ID NO:1 to nucleic acid material of a biological sample, thereby forming a hybridization complex;
- and
- detecting the hybridization complex.
A method of detecting a polypeptide comprising the amino acid sequence shown in SEQ ID NO:2, comprising the steps of:
- contacting a biological sample with a reagent that specifically binds to the polypeptide to form a reagent-polypeptide complex;
- and
- detecting the reagent-polypeptide complex.
A method of screening for agents which can modulate the activity of a human PGC-1-like protein, comprising the steps of:
- contacting a test compound with a polypeptide comprising an amino acid sequence selected from the group consisting of:
- (1) amino acid sequences which are at least about 32% identical to the amino acid sequence shown in SEQ ID NO:2 and (2) the amino acid sequence shown in SEQ ID NO:2;
- and
- detecting binding of the test compound to the polypeptide, wherein a test compound which binds to the polypeptide is identified as a potential agent for regulating activity of the human PGC-1-like protein.
A method of screening for agents which modulate an activity of a human PGC-1-like protein, comprising the steps of:
- contacting a test compound with a polypeptide comprising an amino acid sequence selected from the group consisting of:
- (1) amino acid sequences which are at least about 32% identical to the amino acid sequence shown in SEQ ID NO:2 and (2) the amino acid sequence shown in SEQ ID NO:2;
- and
- detecting an activity of the polypeptide, wherein a test compound which increases the activity of the polypeptide is identified as a potential agent for increasing the activity of the human PGC-1-like protein, and wherein a test compound which decreases the activity of the polypeptide is identified as a potential agent for decreasing the activity of the human PGC-1-like protein.
A method of screening for agents which modulate an activity of a human PGC-1-like protein, comprising the steps of:
- contacting a test compound with a product encoded by a polynucleotide which comprises the nucleotide sequence shown in SEQ ID NO:1;
- and
- detecting binding of the test compound to the product, wherein a test compound which binds to the product is identified as a potential agent for regulating the activity of the human PGC-1-like protein.
A method of reducing activity of a human PGC-1-like protein, comprising the step of:
- contacting a cell with a reagent which specifically binds to a product encoded by a polynucleotide comprising the nucleotide sequence shown in SEQ ID NO:1, whereby the activity of a human PGC-1-like protein is reduced.
A pharmaceutical composition, comprising:
- a reagent which specifically binds to a polypeptide comprising the amino acid sequence shown in SEQ ID NO:2;
- and
- a pharmaceutically acceptable carrier.
A pharmaceutical composition, comprising:
- a reagent which specifically binds to a product of a polynucleotide comprising the nucleotide sequence shown in SEQ ID NO:1;
- and
- a pharmaceutically acceptable carrier.
A pharmaceutical composition, comprising:
- an expression vector encoding a polypeptide comprising the amino acid sequence shown in SEQ ID NO:2;
- and
- a pharmaceutically acceptable carrier.
A method of treating a PGC-1-like protein dysfunction related disease, wherein the disease is selected from cancer, obesity, a cardiovascular disorder, diabetes, or COPD comprising the step of:
- administering to a patient in need thereof a therapeutically effective dose of a reagent that modulates a function of a human PGC-1-like protein, whereby symptoms of the PGC-1-like protein dysfunction related disease are ameliorated.
Dependencies are inferred from the claim text.