Human Pellino polypeptides
AssigneeImmunex Corporation
InventorsTimothy A. Bird, David J. Cosman, Xiaoxia Li
Life of this patent
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Abstract
There are disclosed novel polypeptides referred to as Pellino polypeptides, as well as fragments thereof, including immunogenic peptides. DNAs encoding such polypeptides as well as methods of using such DNAs and polypeptides are also disclosed.
Claims20
A method for identifying compounds that inhibit NF-kB-dependent transcription or p38-dependent transcription comprising:
- (a) mixing a test compound with a Pellino-1 polypeptide;
- (b) assaying the association of said Pellino-1 polypeptide with a binding partner in the presence of said test compound;
- and
- (c) determining whether the test compound inhibits the association of said Pellino-1 polypeptide with a binding partner relative to association of said Pellino-1 polypeptide with said binding partner in the absence of said test compound;
- wherein said Pellino-1 polypeptide comprises an amino acid sequence selected from the group consisting of:
- SEQ ID NO:4;
- amino acids ×1 to ×2 of SEQ ID NO:4, wherein ×1 is any of amino acids 130 through 134 of SEQ ID NO:4, and ×2 is any of amino acids 187 through 191 of SEQ ID NO:4;
- and amino acids ×1 to ×2 of SEQ ID NO:4, wherein ×1 is any of amino acids 1 through 10 of SEQ ID NO:4, and ×2 is any of amino acids 409 through 418 of SEQ ID NO:4;
- and
- wherein the binding partner is selected from the group consisting of IRAK, IRAK-4, and TRAF6.
An inhibitory nucleic acid that binds to a nucleic acid encoding an amino acid sequence selected from the group consisting of:
- (a) SEQ ID NO:4;
- (b) amino acids ×1 to ×2 of SEQ ID NO:4, wherein ×1 is any of amino acids 130 through 134 of SEQ ID NO:4, and ×2 is any of amino acids 187 through 191 of SEQ ID NO:4;
- and
- (c) amino acids ×1 to ×2 of SEQ ID NO:4, wherein ×1 is any of amino acids 1 through 10 of SEQ ID NO:4, and ×2 is any of amino acids 409 through 418 of SEQ ID NO:4;
- wherein the presence of said inhibitory nucleic acid within a cell inhibits the association of Pellino-1 with a binding partner selected from the group consisting of IRAK, IRAK-4, and TRAF6.
An inhibitory polypeptide comprising an antibody fragment that binds to a polypeptide comprising an amino acid sequence selected from the group consisting of:
- (a) SEQ ID NO:4;
- (b) amino acids ×1 to ×2 of SEQ ID NO:4, wherein ×1 is any of amino acids 130 through 134 of SEQ ID NO:4, and ×2 is any of amino acids 187 through 191 of SEQ ID NO:4;
- and
- (c) amino acids ×1 to ×2 of SEQ ID NO:4, wherein ×1 is any of amino acids 1 through 10 of SEQ ID NO:4, and ×2 is any of amino acids 409 through 418 of SEQ ID NO:4;
- wherein the presence of said inhibitory polypeptide within a cell inhibits the association of Pellino-1 with a binding partner selected from the group consisting of IRAK, IRAK-4, and TRAF6.
Dependencies are inferred from the claim text.