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OwnerBruno Tocque, Laurent Bracco +2inventors
InventorsBruno Tocque, Laurent Bracco, Florence Edon, Fabien Schweighoffer
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The invention concerns a method for identifying and/or cloning nucleic acid regions representing qualitative differences associated with alternative splicing events and/or with insertions, deletions located in RNA transcribed genome regions, between two physiological situations, comprising either hybridization of RNA derived from the test situation with cDNA's derived from the reference situation and/or reciprocally, or double-strand hybridization of cDNA derived from the test situation with cDNA's derived from the reference situation; and identifying and/or cloning nucleic acids representing qualitative differences. The invention also concerns compositions or banks of nucleic acids representing qualitative differences between two physiological situations, obtainable by the above method, and their use as probe, for identifying genes or molecules of interest, or still for example in methods of pharmacogenomics, and profiling of molecules relative to their therapeutic and/or toxic effects. The invention further concerns the use of dysregulation of splicing RNA as markers for predicting molecule toxicity and/or efficacy, and as markers in pharmacogenomics.
A method for identifying or cloning nucleic acids comprising sequences corresponding to portions of genes that are differentially spliced between two biological samples containing nucleic acids, wherein the composition or sequence of the nucleic acids in at least one of said biological samples is at least partially unknown, said method comprising:
A method for producing a array of nucleic acids, said method comprising:
A method of producing an array of splice oligonucleotides, comprising:
A product comprising, immobilized on a support material, a plurality of oligonucleotides, wherein (i) said oligonucleotides comprise a sequence that is complementary to and specific for an exon-exon or an exon-intron junction region of a gene or RNA, (ii) said oligonucleotides have a length of between 5 and 100 nucleotides, and (iii) said product comprises at least two sets of oligonucleotides complementary to and specific for a distinct exon-exon or exon-intron junction region of the same gene or RNA,
Dependencies are inferred from the claim text.